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			<p class="Type-of-Article"><span class="CharOverride-1">Research Article</span></p>
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			<p class="title- ParaOverride-1">&nbsp;</p>
			<p class="title- ParaOverride-1">Fertility of Commercial Sexed Semen and the Economic Analyses of its Application in Holstein Heifers</p>
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			<p class="authors-new ParaOverride-1">&nbsp;</p>
			<p class="authors-new ParaOverride-1"><span lang="en-US">Hany Abdalla</span><span class="CharOverride-2" lang="en-US">1</span><span lang="en-US">, Mohamed Abd El-latif Ali</span><span class="CharOverride-2" lang="en-US">2</span><span lang="en-US">, Mahmoud Salah El-Tarabany</span><span class="CharOverride-2" lang="en-US">2</span><span class="CharOverride-3" lang="en-US">  </span>   </p>
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			<p class="Normal" lang="en-US"><span class="CharOverride-5">1</span><span class="CharOverride-6">Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Egypt; </span><span class="CharOverride-5">2</span><span class="CharOverride-6">Department of Animal Wealth Development, Faculty of Veterinary Medicine, Zagazig University, Egypt.</span></p>
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			<p class="Abstract ParaOverride-1">&nbsp;</p>
			<p class="Abstract ParaOverride-1"><span class="CharOverride-4">Abstract</span> | The aim of the current study was to evaluate the fertility of commercially available sexed semen and to economically analyse three assumed strategies for its application in Holstein heifers. In the first part of the study, a total of 426 heifers were inseminated with sexed semen from 7 bulls and 325 heifers were inseminated with unsexed semen from 5 bulls. The pregnancy at 40 and 90 days post insemination, the embryonic loss, the calving, the abortion and the heifer calves rates were calculated. Heifers inseminated with sexed semen had significantly lower (P&lt;0.001) pregnancy-40, pregnancy- 90 and calving rates (34, 32.2 and 29.3%; respectively) than those inseminated with unsexed semen (62.5, 57.8 and 51.1%; respectively). The embryonic loss and abortion rates were similar in heifers inseminated with sexed and unsexed semen. There was a clear effect for bull on pregnancy, embryonic loss and abortion rates in sexed and unsexed semen. In the second part of the study, it was assumed that heifers will be inseminated with sexed semen once, twice or repeatedly till achieving 88% calving rate. The economic impact of the three sexed semen application strategies was compared to the unsexed strategy. The sexed once, the sexed twice and the sexed wide strategies achieved 8.7, 8.1 and 13.8% increase in the calf crop return; respectively. In conclusion, even with lower fertility, application of sexed semen could add numerous benefits to the dairy herds.</p>
		  <p class="Abstract ParaOverride-1">&nbsp;</p>
			<p class="Abstract ParaOverride-1"><span class="CharOverride-4">Keywords</span> | Sexed semen, Reproductive performance, Economic analyses</p>
		  <p class="Abstract ParaOverride-1">&nbsp;</p>
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			<p class="Editor----Citation"><span class="CharOverride-9">Editor</span> | Kuldeep Dhama, Indian Veterinary Research Institute, Uttar Pradesh, India.</p>
			<p class="Editor----Citation"><span class="CharOverride-4">Received</span> | October 15, 2014; <span class="CharOverride-4">Revised</span> | October 23, 2014; <span class="CharOverride-4">Accepted</span> | October 24, 2014; <span class="CharOverride-4">Published</span> | October 30, 2014&#9;&#9;</p>
			<p class="Editor----Citation"><span class="CharOverride-4">*Correspondence</span> | Hany Abdalla , Zagazig University, Egypt; <span class="CharOverride-4">Email</span>: hlabdallah@zu.edu.eg</p>
			<p class="Editor----Citation"><span class="CharOverride-4">Citation</span> | Abdalla H, Ali MA, El-Tarabany MS (2014). Fertility of commercial sexed semen and the economic analyses of its application in Holstein heifers. Adv. Anim. Vet. Sci. 2 (9): 535-542.  </p>
			<p class="Editor----Citation"><span class="CharOverride-9">DOI</span> | <a href="http://dx.doi.org/10.14737/journal.aavs/2014/2.9.535.542"><span class="Hyperlink">http://dx.doi.org/10.14737/journal.aavs/2014/2.9.535.542</span></a></p>
			<p class="Editor----Citation"><span class="Editor---Citation CharOverride-4" lang="en-US">ISSN </span><span class="Editor---Citation CharOverride-4" lang="en-US">(</span><span class="Editor---Citation CharOverride-4" lang="en-US">Online</span><span class="Editor---Citation CharOverride-4" lang="en-US">)</span> | 2307-8316; <span class="Editor---Citation CharOverride-4" lang="en-US">ISSN </span><span class="Editor---Citation CharOverride-4" lang="en-US">(Print) </span> | 2309-3331</p>
			<p class="Editor----Citation"><span class="CharOverride-9">Copyright </span>© 2014 Abdalla et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p>
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			<p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-1--Introduction----">&nbsp;</p>
		  <p class="Heading-1--Introduction----">INTRODUCTION </p>
			<p class="Caps-on-First-Para">&nbsp;</p>
			<p class="Caps-on-First-Para"><span class="_idGenDropcap-1">S</span>exed semen is a new reproductive technology aimed to alter the sex ratio of the offspring toward a desired gender. About 90% of the new born will be from the required sex (DeJarnette et al., 2009). Bovine sexed semen was introduced into commercial application for the first time in the United Kingdom in 2000. Now a days, large number of artificial insemination centres offered bovine sexed semen worldwide (Rath and Johnson, 2008).The application of sexed semen extended to different species (Rath and Johnson, 2008; Lu et al., 2010) and it began to be used with other advanced reproductive techniques such as IVF (Pontes et al., 2010) and super ovulation (Hayakawa et al., 2009). </p>
		  <p class="Caps-on-First-Para">&nbsp;</p>
			<p class="Heading-1--Introduction---- ParaOverride-1"><span class="CharOverride-12">In expanding closed dairy herds, application of sexed semen facilitates faster, more profitable expansion of the herds. While in non-expanding dairy herds, sexed semen may be applied for production of replacement heifers for sale or to increase the value of beef output from the dairy herds (Hohenboken, 1999). Additionally, application of sexed semen could be an effective tool to improve the annual genetic gain (Sørensen et al., 2011; Khalajzadeh et al., 2012) through production of replacement heifers only from the superior genetics cows or higher chance to select the best genetic potential as replacement heifers (Sørensen et al., 2011; Khalajzadeh et al., 2012). </span></p>
		  <p class="Heading-1--Introduction---- ParaOverride-1">&nbsp;</p>
			<p class="Heading-1--Introduction---- ParaOverride-1"><span class="CharOverride-12">Limited application of sexed semen in developing countries may be attributed to its high price and low fertility (Seidel, 2003). The price of one sexed insemination dose is four times as the price of the unsexed one. The recorded sexed semen conception rate differed from herd to herd (DeJarnette et al., 2009) and from breed to another (Borchersen and Peacock, 2009). However, the cumulative data stated that the fertility of sexed semen is lower than that of unsexed one (Borchersen and Peacock, 2009; DeJarnette et al., 2009; Dominguez et al., 2011). About two-thirds of sexed semen fertility reduction was refereed to lower sperm number in the insemination dose (2-4 million), and the other third was refereed to the adverse effects of the sorting procedures (Frijters et al., 2009). The lower fertility limited the application of sexed semen to heifers those have higher predicted fertility (DeJarnette et al., 2009). This application may be limited to the first insemination or may be repeated to more inseminations. However, some recent reports recorded similar pregnancy rate after using sexed and non-sexed semen in Holstein heifers (An et al., 2010), non-suckling Nelore cows (Dominguez et al., 2011) buffalo heifers (Campanile et al., 2011), buffalo-cows (Lu et al., 2010; Campanile et al., 2013).</span></p>
		  <p class="Heading-1--Introduction---- ParaOverride-1">&nbsp;</p>
			<p class="Heading-1--Introduction---- ParaOverride-1"><span class="CharOverride-12">Despite of higher price and lower fertility, application of sexed semen had resulted in greater net profit than the unsexed semen at the end of time horizon (Hossein-Zadeh et al., 2010). The sexed semen conception rate and the extent of sexed semen application within the herd greatly affect the economic impact of sexed semen application. Therefore, the present study was designed to investigate the fertility of commercially available sexed semen and to economically analyse three assumed strategies for sexed semen application in Holstein heifers under Egyptians conditions. </span></p>
			<p class="Heading-1--Introduction---- ParaOverride-1">&nbsp;</p>
		  <p class="Heading-1--Introduction---- ParaOverride-1">&nbsp;</p>
			<p class="Heading-1--Introduction----">MATERIALS AND METHODS </p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Heifer’s Management and Examination</span>  </p>
			<p class="Heading-2--History-in-MM- ParaOverride-1"><span class="CharOverride-12">This study was carried out in ALQasem farm, Ismailia Road, Egypt in the period from September 2010 to December 2013. A number of 751 Nulliparous Holstein heifers were included in this study. Heifers were housed in a free stall and had a free access to water and feed ad libitum a total mixed well balanced ration. At 12 months age, the wither height and body weight were recorded. Heifers have body weight over 350 kg and wither height over 125 cm were judged as a mature heifers. All heifers were examined gynaecologically to exclude any genital tract anomalies. Heifers passed the gynaecological and body measure examination were proved for breeding. </span></p>
		  <p class="Heading-2--History-in-MM- ParaOverride-1">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Semen</span> </p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB">All unsexed and sexed semen used in the current study were imported from American Breeding Service (ABS Global, Inc., Wisconsin, USA). Unsexed semen from 5 bulls (H,J,K,L,M) and sexed semen from 7 bulls (A,B,C,D,E,F,G) were used in the current study. According to the ABS sexation technical guide, the pre-freeze sexed sperm cell concentration is 2.1 million cells, and on average there are roughly one million motile sperm cells at thawing. While pre-freeze sperm cell concentration in unsexed semen was 25 million. </span></p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-17" lang="en-GB"> </span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Estrus Detection and Artificial Insemination</span></p>
			<p class="Heading-2--History-in-MM- ParaOverride-1"><span class="CharOverride-12">Heifers were observed by professional heat observer all over the day. Heifers that showed standing estrus were introduced for examination 10-14 h later according to the AM/PM rules. Upon examination, heifers that showed turgid uterus and/or estrous mucus were inseminated either with unsexed or sexed semen. The semen was deposited into the uterine body by three skilled professional inseminators.  </span></p>
		  <p class="Heading-2--History-in-MM- ParaOverride-1">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Pregnancy Diagnosis </span></p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB">The non-returned heifers were examined for pregnancy diagnosis at 40 days post-insemination and confirmatory pregnancy diagnosis was carried out at 90 days post-insemination. The pregnancy-40 and pregnancy-90 rates were calculated as the number of heifers diagnosed as pregnant at 40 and 90 days post-insemination -respectively- divided by the number of inseminated heifers. The embryonic loss rate was calculated as the number of heifers diagnosed as non-pregnant at day 90 divided by the number of pregnant heifers at day 40. The calving rate was calculated as the number of heifers gives birth after a complete pregnancy period divided by the number of inseminated heifers. The abortion rate was calculated as the number of heifers diagnosed as non-pregnant after confirmed pregnant at day 90 divided by the number of heifers diagnosed pregnant at day 40. </span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Economic Evaluation </span></p>
			<p class="Heading-2--History-in-MM- ParaOverride-1"><span class="CharOverride-12">Using the actual fertility indices of the commercially available sexed and unsexed semen, three assumed strategies for sexed semen application in heifers were economically analysed in comparison to unsexed strategy. In the first strategy (sexed once), it was assumed that heifers will be inseminated once with sexed semen. In the second strategy (sexed twice), it was assumed that heifers will be inseminated twice with sexed semen. In the first and second strategies, non-pregnant heifers after insemination with sexed semen will be inseminated with unsexed semen till achieving 88 % calving. In the third strategy (repeated sexed), it was assumed that heifers will be repeatedly inseminated with sexed semen till achieving 88% calving. In the unsexed strategy, it was assumed that heifers will be inseminated with unsexed semen till achieving 88% calving.  </span></p>
		  <p class="Heading-2--History-in-MM- ParaOverride-1">&nbsp;</p>
			<p class="Heading-2--History-in-MM- ParaOverride-1"><span class="CharOverride-12">The number of heifers in all strategies was fixed to 100. The actual calving rate and the actual heifer calves ratio after sexed and unsexed semen were used to calculate the number of insemination required to achieve 88% calving and the suspected number of heifer calves in each strategy. The period between inseminations was adjusted to 30 days and it was used to calculate the suspected age at first calving and the suspected extra cost for rearing the heifers for longer period before the first calving. </span></p>
		  <p class="Heading-2--History-in-MM- ParaOverride-1">&nbsp;</p>
			<p class="Heading-2--History-in-MM- ParaOverride-1"><span class="CharOverride-12">Since the cost of rearing heifers from birth till breeding age is constant, only the cost of rearing heifers from breeding age till the first calving was taken in consideration. The suspected costs of insemination and rearing heifers for prolonged period in all sexed semen application strategies were compared to those suspected in the unsexed strategy. The suspected return from calf crop (pregnant heifers or fattened males) was calculated for all strategies. The percent of gain or loss after different sexed semen application strategies in comparison to unsexed strategy was calculated according to the following equations. </span></p>
		  <p class="Heading-2--History-in-MM- ParaOverride-1">&nbsp;</p>
			<p class="Heading-2--History-in-MM- ParaOverride-1"><span class="CharOverride-19">   </span></p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-20" lang="en-GB">Return from heifers</span><span class="CharOverride-16" lang="en-GB">= Suspected number of heifers * price of the heifer in Egyptian pounds (LE).</span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-20" lang="en-GB">Return from males</span><span class="CharOverride-16" lang="en-GB"> = Suspected number of males * price of the male (LE)</span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-20" lang="en-GB">Insemination costs</span><span class="CharOverride-16" lang="en-GB"> = suspected number of required inseminations * price of the straw.</span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-20" lang="en-GB">Additional costs from rearing heifers</span><span class="CharOverride-16" lang="en-GB"> = Price of daily feed intake * days between each in two successive insemination. </span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-20" lang="en-GB">Total return from the strategy </span><span class="CharOverride-16" lang="en-GB">= (Return from heifers + Return from calves) – (insemination costs + additional costs from rearing heifers)</span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Statistical Analysis</span> </p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB">All statistical procedures were performed using SAS statistical system Package V9.2 (SAS, 2002).The proportion of dichotomous variables (pregnancy-40, pregnancy-90, embryonic loss, abortion, heifers calves) was evaluated with Chi-square test. Significant results were followed by multiple Z-tests to compare corresponding proportions. P-value for all pairwise comparisons was adjusted using the Bonferroni correction.</span></p>
			<p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Heading-1--Introduction----">RESULTS </p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Fertility of Sexed Semen</span> </p>
			<p class="Normal ParaOverride-1" lang="en-US"><span class="CharOverride-16" lang="en-GB">Heifers inseminated with sexed semen had significantly lower (P &lt;0.001) pregnancy-40, pregnancy- 90 and calving rates (34, 32.2 and 29.3 %; respectively) than those inseminated with unsexed semen (62.5, 57.8 and 51.1%; respectively). Similar embryonic loss and abortion rates were recorded in heifers inseminated with sexed (5.5 and 8.3 %; respectively) and unsexed semen (7.4 and 10.8%, respectively). The proportion of heifers calves after using sexed semen (89.6) was significantly higher than that after using unsexed semen (50; P &lt;0.001) (Table 1).</span></p>
		  <p class="Normal ParaOverride-1" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-1" lang="en-US"><span class="CharOverride-16" lang="en-GB">The pregnancy-40, the pregnancy-90 and the calving rates were variable among different bulls in the unsexed (55.3 to 69.3, 51.6 to 66.7 and 44.7 to 56.1; respectively) and sexed (27.4 to 43.4 and 24.2 to 40.4 and 22.6 to 37.5; respectively) semen. The differences in pregnancy-40 and pregnancy-90 rates reach a significant level among sexed and unsexed bulls; respectively (P&lt;0.05). The deviation from the average pregnancy-40 ratio was ranged from -6.4 to 9.6 and -1.4 to 8.5 in sexed and unsexed semen; respectively. The embryonic loss and abortion rates were significantly different among unsexed bulls (3.8 to 13.3 and 1.9 to 15.2; respectively) and sexed bulls (0 to 11.8 and 0 to 14; respectively).  The sexed semen from bull B gave a significantly lower heifers calves ratio than other bulls (Table 2, 3).</span></p>
			<p class="Figure--and-Table-Heading">&nbsp;</p>
			<p class="Figure--and-Table-Heading">&nbsp;</p>
		  <p class="Figure--and-Table-Heading"><span class="CharOverride-4">Table 1:  </span><span class="CharOverride-21">Reproductive performance of heifers inseminated with sexed and unsexed semen</span></p>
			<table width="657" height="74" class="Table-Style-1" id="table-1">
				<colgroup>
					<col class="_idGenTableRowColumn-1" />
					<col class="_idGenTableRowColumn-2" />
					<col class="_idGenTableRowColumn-3" />
					<col class="_idGenTableRowColumn-3" />
					<col class="_idGenTableRowColumn-4" />
					<col class="_idGenTableRowColumn-1" />
					<col class="_idGenTableRowColumn-5" />
					<col class="_idGenTableRowColumn-6" />
				</colgroup>
				<tbody>
					<tr class="_idGenTableRowColumn-7">
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Semen</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Insemination</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Preg-40</span><span class="Title CharOverride-24">1</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Preg-90</span><span class="Title CharOverride-24">1</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Embryonic loss</span><span class="Title CharOverride-24">2</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Calving</span><span class="Title CharOverride-24">1</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Abortion</span><span class="Title CharOverride-24">3</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Heifer calves</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-8">
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Sexed</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">426</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">34</span><span class="Title CharOverride-2">b</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">32.2</span><span class="Title CharOverride-2">b</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">5.5</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">29.3</span><span class="Title CharOverride-2">b</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">8.3</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">89.6</span><span class="Title CharOverride-2">a</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-9">
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title CharOverride-4">Unsexed</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">325</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">62.5</span><span class="Title CharOverride-2">a</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">57.8</span><span class="Title CharOverride-2">a</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">7.4</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">51.1</span><span class="Title CharOverride-2">a</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">10.8</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-4"><span class="Title">50</span><span class="Title CharOverride-2">b</span></p>
						</td>
					</tr>
				</tbody>
			</table>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-25">Values with different superscript (a, b) at the same column are significantly different (P&lt;0.001). All values were presented as proportions; </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">1</span><span class="CharOverride-25">Pregnancy-40, Pregnancy-90 and calving rates were calculated as the number of heifers diagnosed as pregnant at 40 and 90 days post-insemination and number of heifers give birth after a complete pregnancy period respectively divided by the number of inseminated heifers; </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">2</span><span class="CharOverride-25">The embryonic loss rate was calculated as the number of heifers diagnosed as non-pregnant at day 90 divided by the number of pregnant heifers at day 40; </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">3</span><span class="CharOverride-25">The abortion rate was calculated as the number of heifers diagnosed as non-pregnant after confirmed pregnant at day 90 divided by the number of heifers diagnosed pregnant at day 40. </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
		  <p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-4">Table 2:  </span><span class="CharOverride-21">Effect of bull on reproductive performance of heifers inseminated with unsexed semen </span></p>
			<table width="657" height="144" class="Table-Style-1" id="table-2">
				<colgroup>
					<col class="_idGenTableRowColumn-10" />
					<col class="_idGenTableRowColumn-2" />
					<col class="_idGenTableRowColumn-11" />
					<col class="_idGenTableRowColumn-11" />
					<col class="_idGenTableRowColumn-4" />
					<col class="_idGenTableRowColumn-12" />
					<col class="_idGenTableRowColumn-13" />
					<col class="_idGenTableRowColumn-14" />
				</colgroup>
				<tbody>
					<tr class="_idGenTableRowColumn-15">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Bull</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Insemination</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Preg-40</span><span class="Title CharOverride-29">1</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Preg-90</span><span class="Title CharOverride-29">1</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Embryonic loss</span><span class="Title CharOverride-29">2</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Calving</span><span class="Title CharOverride-29">1</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Abortion</span><span class="Title CharOverride-29">3</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">Heifer calves</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-16">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">H</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">114</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">69.3</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">66.7</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">3.8</span><span class="Title CharOverride-32"> b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">56.1</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">15.2</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">50</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-17">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">J</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">93</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">58.1</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">51.6</span><span class="Title CharOverride-32"> b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">11.1</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">50.5</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">1.9</span><span class="Title CharOverride-32"> b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">48.9</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-16">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">K</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">38</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">55.3</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">52.6</span><span class="Title CharOverride-32"> a b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">4.8</span><span class="Title CharOverride-32"> a b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">44.7</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">14.3</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">47.1</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-17">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">L</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">48</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">62.3</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">54.2</span><span class="Title CharOverride-32"> a b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">13.3</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">45.8</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">13.3</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">50</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-16">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-28">M</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">32</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">59.4</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">65.3</span><span class="Title CharOverride-32"> a</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">5.3</span><span class="Title CharOverride-32"> a b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">50</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">10.5</span><span class="Title CharOverride-32"> a b</span></p>
						</td>
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-31">56.3</span></p>
						</td>
					</tr>
				</tbody>
			</table>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-25">Values with different superscript (a, b) at the same column are significantly different (P&lt;0.05).  All values were presented as proportions; </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">1</span><span class="CharOverride-25">Pregnancy-40, Preganncy-90 and calving rates were calculated as the number of heifers diagnosed as pregnant at 40 and 90 days post-insemination and number of heifers give birth after a complete pregnancy period respectively divided by the number of inseminated heifers; </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-33">2</span><span class="CharOverride-25">The embryonic loss rate was calculated as the number of heifers diagnosed as non-pregnant at day 90 divided by the number of pregnant heifers at day 40; </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">3</span><span class="CharOverride-25">The abortion rate was calculated as the number of heifers diagnosed as non-pregnant after confirmed pregnant at day 90 divided by the number of heifers diagnosed pregnant at day 40. </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
		  
          <p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-4">Table 3: </span><span class="CharOverride-21">Effect of bull on reproductive performance of heifers inseminated with sexed semen</span></p>
          
			
		  <table width="657" height="186" class="Table-Style-1" id="table-3">
				<colgroup>
					<col class="_idGenTableRowColumn-18" />
					<col class="_idGenTableRowColumn-19" />
					<col class="_idGenTableRowColumn-5" />
					<col class="_idGenTableRowColumn-20" />
					<col class="_idGenTableRowColumn-5" />
					<col class="_idGenTableRowColumn-21" />
					<col class="_idGenTableRowColumn-22" />
					<col class="_idGenTableRowColumn-23" />
				</colgroup>
				<tbody>
					<tr class="_idGenTableRowColumn-7">
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-20">Bull</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-20">Insemination</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-20">Preg-40</span><span class="Title CharOverride-39">1</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-20">Preg-90</span><span class="Title CharOverride-39">1</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-20">Embryonic loss</span><span class="Title CharOverride-39">2</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-6" lang="en-US"><span class="Title CharOverride-20">Calving</span><span class="Title CharOverride-39">1</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-7" lang="en-US"><span class="Title CharOverride-20">Abortion</span><span class="Title CharOverride-39">3</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-7" lang="en-US"><span class="Title CharOverride-20">Heifer calves</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-24">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">A</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">48</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">37.5</span><span class="Title CharOverride-40"> ab</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">37.5</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">0</span><span class="Title CharOverride-40">b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">35.4</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">5.6</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">100</span><span class="Title CharOverride-40">a</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-25">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">B</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">97</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">28.9</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">28.9</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">0</span><span class="Title CharOverride-40">b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">27.8</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">2.6</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">59.3</span><span class="Title CharOverride-40"> b</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-24">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">C</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">99</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">43.4</span><span class="Title CharOverride-40"> a</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">40.4</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">6.8</span><span class="Title CharOverride-40"> ab</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">34.3</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">14</span><span class="Title CharOverride-40"> a</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">91.2</span><span class="Title CharOverride-40"> a</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-25">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">D</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">57</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">29.8</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">26.3</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">11.8</span><span class="Title CharOverride-40"> a</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">22.8</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">11.8</span><span class="Title CharOverride-40"> ab</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">100</span><span class="Title CharOverride-40"> a</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-24">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">E</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">62</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">27.4</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">24.2</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">11.8</span><span class="Title CharOverride-40"> a</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">22.6</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">5.9</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">100</span><span class="Title CharOverride-40"> a</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-26">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">F</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">32</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">40.6</span><span class="Title CharOverride-40"> a</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">37.5</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">7.7</span><span class="Title CharOverride-40"> ab</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">37.5</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">0 </span><span class="Title CharOverride-40">b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">100</span><span class="Title CharOverride-40">a</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-27">
						<td>
							<p class="Normal" lang="en-US"><span class="Title CharOverride-20">G</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">31</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">29.1</span><span class="Title CharOverride-40"> b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">29.1</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">0</span><span class="Title CharOverride-40">b</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">25.8</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">11.1</span><span class="Title CharOverride-40"> ab</span></p>
						</td>
						<td>
							<p class="Normal ParaOverride-5" lang="en-US"><span class="Title CharOverride-16">100</span><span class="Title CharOverride-40"> a</span></p>
						</td>
					</tr>
				</tbody>
			</table>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-25">Values with different superscript (a, b) at the same column are significantly different (P &lt;0.05).  All values were presented as proportions  </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">1</span><span class="CharOverride-25">Pregnancy-40, Preganncy-90 and calving rates were calculated as the number of heifers diagnosed as pregnant at 40 and 90 days post-insemination and number of heifers give birth after a complete pregnancy period respectively divided by the number of inseminated heifers</span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">2</span><span class="CharOverride-25">The embryonic loss rate was calculated as the number of heifers diagnosed as non-pregnant at day 90 divided by the number of pregnant heifers at day 40</span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">3</span><span class="CharOverride-25">The abortion rate was calculated as the number of heifers diagnosed as non-pregnant after confirmed pregnant at day 90 divided by the number of heifers diagnosed pregnant at day 40. </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
		  <p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-4">Table 4: </span><span class="CharOverride-21">Economic analysis for different sexed semen application strategies</span></p>
			<table width="657" class="Table-Style-1" id="table-4">
				<colgroup>
					<col class="_idGenTableRowColumn-28" />
					<col class="_idGenTableRowColumn-29" />
					<col class="_idGenTableRowColumn-30" />
					<col class="_idGenTableRowColumn-30" />
					<col class="_idGenTableRowColumn-31" />
				</colgroup>
				<tbody>
					<tr class="_idGenTableRowColumn-9">
						<td rowspan="2" />
						<td rowspan="2">
							<p class="Body-Text ParaOverride-1"><span class="Title">Unsexed</span><span class="Title CharOverride-2">1</span></p>
						</td>
						<td colspan="3">
							<p class="Body-Text ParaOverride-1"><span class="Title">Sexed semen</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-8">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Sexed once</span><span class="Title CharOverride-2">2</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Sexed twice</span><span class="Title CharOverride-2">3</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Sexed wide </span><span class="Title CharOverride-2">4</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-25">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">No. of sexed straw*</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">0</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">100</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">171</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">298</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-27">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">No. of unsexed straw*</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">173</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">122</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">74</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">0</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-25">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Cost of insemination</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">8640</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">26108</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">37861</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">59734</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-27">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Increase in insemination cost**  </span></p>
						</td>
						<td />
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">2</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">4.4</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">6.9</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-25">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Age at first calving (month)</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">22.6 ± 0.73</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">22.9 ± 0.83</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">23.2 ± 1.1</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">23.5 ± 1.5</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-27">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Extra cost for rearing ***</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">22935</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">38486</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">45715</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">62582</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-32">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Increase in the rearing cost** </span></p>
						</td>
						<td />
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">1.7</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">2</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">2.7</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-27">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Number of heifer calves</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">44</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">56</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">64</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">80</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-25">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Number of bull calves</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">44</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">32</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">24</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">8</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-24">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Net return from calf crop</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">1734563</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">1886093</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">1875434</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">1974454</span></p>
						</td>
					</tr>
					<tr class="_idGenTableRowColumn-33">
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">Gain (loss) of sexed semen application**</span></p>
						</td>
						<td />
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">+ 8.7</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">+ 8.1</span></p>
						</td>
						<td>
							<p class="Body-Text ParaOverride-1"><span class="Title">+ 13.8</span></p>
						</td>
					</tr>
				</tbody>
			</table>
			<p class="Normal" lang="en-US">&nbsp;</p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-25">N.B. Costs and returned were calculated by Egyptian pound </span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">1</span><span class="CharOverride-25">Unsexed: Heifers were inseminated with unsexed semen till achieving 88% pregnancy</span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">2</span><span class="CharOverride-25"> Sexed once: Heifers inseminated once with sexed semen then non-pregnant heifers after insemination with sexed semen were inseminated with unsexed semen till achieving 88 % pregnancy</span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">3</span><span class="CharOverride-25">Sexed twice: Heifers inseminated twice with sexed semen then non-pregnant heifers after insemination with sexed semen were inseminated with unsexed semen till achieving 88 % pregnancy</span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-26">4</span><span class="CharOverride-25">Sexed wide: Heifers repeatedly inseminated with sexed semen till achieving 88% pregnancy</span></p>
			<p class="Figure--and-Table-Heading ParaOverride-1"><span class="CharOverride-25">*Number</span><span class="CharOverride-26"> </span><span class="CharOverride-25">of straws required to achieve 88% pregnancy; **Calculated as comparison to the unsexed strategy; ***Extra coast for rearing from breeding age till first calving due to lower fertility     </span></p>
		  <p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
		  <p class="Figure--and-Table-Heading ParaOverride-1">&nbsp;</p>
			
            <p class="Heading-2--History-in-MM-"><span class="CharOverride-14">Economic analysis of sexed semen application </span></p>
			<p class="Normal ParaOverride-1" lang="en-US"><span class="CharOverride-16" lang="en-GB">The suspected age at first calving in the unsexed strategy (22.6 ± 0.73 m) was younger than that in different sexed semen application strategies (22.9 to 23.5, 22.9 ± 0.83 to 23.5 ± 1.5 m). The extra coast for rearing in the sexed once, the sexed twice and the wide sexed strategies were 1.7, 2 and 2.7 times as that in the unsexed semen strategy. Additionally, the cost of insemination required for achieving 88% calving in the sexed once, the sexed twice and the wide sexed strategies were 2, 4.4 and 6.9 times as that in the unsexed strategy. The number of heifers calves increased from 44 in the unsexed semen strategy to 56,64 and 80 in sexed once, sexed twice and sexed wide strategies; respectively. The sexed once, the sexed twice and the sexed wide strategies achieved 8.7, 8.1and 13.8% increase in the calf crop return; respectively (Table 4).</span></p>
			<p class="Normal ParaOverride-1" lang="en-US">&nbsp;</p>
		  <p class="Normal ParaOverride-1" lang="en-US">&nbsp;</p>
			<p class="Heading-1--Introduction----">DISCUSSION </p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-1--Introduction---- ParaOverride-1"><span class="CharOverride-12">Under field conditions, it is difficult to compare the fertility of sexed and unsexed semen collected from the same bulls. The current study compared the fertility of commercially available sexed and unsexed semen and economically analysed three assumed strategies for sexed semen application in heifers. </span></p>
		  <p class="Heading-1--Introduction---- ParaOverride-1">&nbsp;</p>
			<p class="Heading-1--Introduction---- ParaOverride-1"><span class="CharOverride-12">Significantly lower sexed semen pregnancy rate recorded in the current study is in agreement with previous studies (Bodmer et al., 2005; Dominguez et al., 2011) but in disagreement with An</span><span class="CharOverride-34"> </span><span class="CharOverride-12">et al. (2010). The sexed semen pregnancy rate recorded in the current study is around 55 % of the unsexed semen one. This is similar to results recorded by Bodmer et al. (2005) using Brown and Red Holstein heifers and some trials of Weigel (2004) and Schenk et al. (2009). However it is comparatively lower than many reports stated that the fertility of sexed semen reached about 75-85 % of the unsexed semen (Garner and Seidel, 2003; DeJarnette et al., 2009; Borchersen and Peacock, 2009) and some trials of Seidel et al. (1999). The lower sexed/unsexed pregnancy proportion recorded in the current study is mainly due to comparatively lower sexed semen pregnancy rate that was about 20-30% lower than previous reports (Seidel et al., 1999; Borchersen and Peacock, 2009; DeJarnette et al., 2009). Since heifers that used in the current study received the same management including nutrition, housing, estrus detection, timing of AI as well as animal health programs and inseminated with the same inseminator. The lower sexed semen pregnancy rate may be due to difference in initial bull fertility or due to improper handling of the sexed semen itself during transportation of the sexed semen between different dealers.</span></p>
		  <p class="Heading-1--Introduction---- ParaOverride-1">&nbsp;</p>
			<p class="Normal ParaOverride-1" lang="en-US"><span class="CharOverride-16" lang="en-GB">Staining with Hoechst and exposure to UV during flow sorting tended to increase the incidence of sperm chromosome aberrations (Libbus et al., 1987). This was thought to affect fertilizing ability and embryonic survival after sexed semen application. In our study, we recoded similar embryonic loss and abortion proportions after using sexed and unsexed semen which is in agreement with some previous studies (Seidel et al., 1999; Borchersen and Peacock, 2009). This may be due to the fact that sperms with compromised DNA are removed during sorting procedures (Blondin et al., 2009; Gosálvez et al.,2011) and/or that the sperm sorting by flow cytometry improves the DNA integrity of the sperm cell population (Boe-Hansen et al., 2005). Bodmer et al. (2005) reported numerically higher proportion of embryonic loss after using sexed semen. However they used considerably few numbers of heifers.</span></p>
		  <p class="Normal ParaOverride-1" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-1" lang="en-US"><span class="CharOverride-16" lang="en-GB">The proportion of heifer calves after application of sexed semen recoded in the current study is in agreement with Bodmer et al. (2005) but lower than other earlier reports (Garner and Seidel, 2003;  DeJarnette et al., 2009; Schenk et al.,2009) which indicates that the accuracy of the sorting procedures applied by the semen origin company is acceptable.  </span></p>
		  <p class="Normal ParaOverride-1" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB">The fertility variations among bulls in the unsexed and sexed groups is in agreement with previous reports (Borchersen and Peacock, 2009; Frijters et al. 2009; Sales et al., 2010). These variations were attributed to different ability to penetrate the genital tract mucus and different ability to fertilize the oocytes (Al Naib et al., 2011) and/or different expression of some functional sperm specific proteins (Kasimanickam et al., 2012). Higher deviation from the average pregnancy-40 ratio among sexed semen bulls than unsexed semen bulls is in agreement with DeJarnette et al. (2009). Sexed semen may tend to magnify sire fertility difference that exist in unsexed semen due to sire variation in threshold sperm number required for optimum fertility (DeJarnette et al., 2009), improper selection of bulls those cannot achieve acceptable fertility with unsorted low dose (Rath and Johnson, 2008) and/or different ability to survive the sorting procedures (Borchersen and Peacock, 2009; Frijters et al., 2009; Schenk et al., 2009). The Clear effect of bull on the embryonic loss and abortion rates either in sexed and unsexed semen is in agreement with previous results (López-Gatius et al., 2002; Pegorer et al., 2007). Some bulls may have some recessive defects that adversely affecting embryo or fatal survival (VanRaden and Miller, 2006). Although most of sexed semen from different bulls achieved considerable high heifers calves ratio, sexed semen from bull B achieved comparatively lower heifers calves ratio. This may indicate a possibility for improper sorting procedures even with imported sexed semen form known company.  </span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB">The fertility of sexed semen in comparison to the unsexed one is one of the important factors that greatly affect the economic impact of sexed semen application. The lower fertility of the sexed semen recorded in the current study resulted in great increase in the number of inseminations required to achieve 88% calving and subsequently increase the costs of insemination and rearing heifers for prolonged time before the first calving in all assumed strategies for sexed semen application. However, even with this higher coasts all sexed semen application strategies investigated in the current study had resulted in higher calf crop gain in comparison to unsexed strategy. The highest gain was recorded in the wide application strategy. Of course if the fertility of sexed semen is improved the economic impact will be maximized.  If we added this economic benefit into the higher ability to expand the closed herd and the higher suspected genetic improvement of the herd, we can conclude that even with lower fertility, application of sexed semen could add numerous benefits to the dairy herds.  </span></p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB"> </span></p>
			<p class="Heading-1--Introduction----">CONFLICT OF INTEREST </p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-1--Introduction----"><span class="CharOverride-12">None of the authors have any conflict of interest to declare.</span></p>
			<p class="Heading-1--Introduction----">&nbsp;</p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-1--Introduction----">AUTHOR CONTRIBUTIONS</p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Normal ParaOverride-2" lang="en-US"><span class="CharOverride-16" lang="en-GB">All authors have been involved in designing the study, analysing the data and drafting the manuscript.</span></p>
			<p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
		  <p class="Normal ParaOverride-2" lang="en-US">&nbsp;</p>
			<p class="Heading-1--Introduction----">ACKNOWLEDGEMENTS</p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-1--Introduction----"><span class="CharOverride-12">The authors wish to thank the owner of AL-Qasem farm, Ismailia road, Cairo for allowing us to collect the data. We greatly appreciated the great help of veterinary doctor Mohamed Hussein the head manger of the farm in collecting and managing the data by the AfiFarm.  </span></p>
			<p class="Heading-1--Introduction----"><span class="CharOverride-12">    </span></p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			<p class="Heading-1--Introduction----">REFERENCES </p>
		  <p class="Heading-1--Introduction----">&nbsp;</p>
			
			  <li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Al Naib A, Hanrahan JP, Lonergan P, Fair S (2011).  In vitro assessment of sperm from bulls of high and low field fertility. Theriogenology 76: 161–167.</span><a href=" http://dx.doi.org/10.1016/j.theriogenology.2010.10.038 "><span class="Hyperlink CharOverride-45"> http://dx.doi.org/10.1016/j.theriogenology.2010.10.038</span></a></li>
              <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">An L, Wu ZH, Wu YF, Zhang XL, Liu X, Zhu YB, Cheng WM, Gao HM, Guo M, Tian JH (2010). Fertility in Single-ovulating and Superovulated Dairy Heifers after Insemination with Low Dose Sex-sorted Sperm. Reprod. Dom. Anim. 45: e344–e350. </span><a href="http://dx.doi.org/10.1111/j.1439-0531.2009.01574.x"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1111/j.1439-0531.2009.01574.x</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Blondin P, Bealieu M, Fournier V, Morin N, Crawford L, Madan P, King WA (2009). Analysis of bovine sexed sperm for IVF from sorting to the embryo. Theriogenology 71: 30–38. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2008.09.017"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2008.09.017</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Bodmer M, Janett F, Ha¨ssig M, den Daas N, Reichert P, Thun R (2005). Fertility in heifers and cows after low dose insemination with sex-sorted and non-sorted sperm under field conditions. Theriogenology 64: 1647–1655. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2005.04.011"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2005.04.011</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Boe-Hansen GB, Morris ID, Ersbøll AK, Greve T, Christensen P (2005). DNA integrity in sexed bull sperm assessed by neutral Comet assay and sperm chromatin structure assay. Theriogenology 63: 1789–1802. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2004.08.004"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2004.08.004</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Borchersen S, Peacock M (2009). Danish A. I. field data with sexed semen. Theriogenology 71: 59–63. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2008.09.026"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2008.09.026</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Campanile G, Gasparrini B, Vecchio D, Neglia G, Senatore EM, Bella A, Presicce GA, Zicarelli L (2011). Pregnancy rates following AI with sexed semen in Mediterranean Italian buffalo heifers (Bubalus bubalis). Theriogenology 76: 500–506.  </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2011.02.029"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2011.02.029</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Campanile G, Vecchio D, Neglia G, Bella A, Prandi A, Senatore EM, Gasparrini B, Presicce GA (2013). Effect of season, late embryonic mortality and progesterone production on pregnancy rates in pluriparous buffaloes (Bubalus bubalis) after artificial insemination with sexed semen. Theriogenology 79: 653–659. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2012.11.020"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2012.11.020</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">DeJarnette JM, Nebel RL, Marshall CE (2009). Evaluating the success of sex-sorted semen in US dairy herds from on farm records. Theriogenology 71: 49–58. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2008.09.042"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2008.09.042</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Dominguez JH, Costa DS, Centurion VJ, Faria FJ (2011). Pregnancy rate of Nelore females inseminated with male-sexed semen. Anim. Reprod. Sci. 129: 127–131. </span><a href="http://dx.doi.org/10.1016/j.anireprosci.2011.11.002"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.anireprosci.2011.11.002</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Frijters ACJ,E. Mullaart E, Roelofs RMG, van Hoorne RP, Moreno JF, Moreno O, Merton JS (2009). What affects fertility of sexed bull semen more, low sperm dosage or the sorting process? Theriogenology 71: 64–67. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2008.09.025"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2008.09.025</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Garner DL, Seidel GE Jr (2003). Past, present and future perspectives on sexing sperm. Can. J. Anim. Sci. 83: 375–384. </span><a href="http://dx.doi.org/10.4141/A03-022 "><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.4141/A03-022</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Gosálvez J, Ramirez MA, López-Fernández C, Crespo F, Evans KM, Kjelland ME,  Moreno JF (2011). Sex-sorted bovine spermatozoa and DNA damage: I. Static features. Theriogenology 75: 197- 205. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2010.08.006"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2010.08.006</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Hayakawa H, Hirai T, Takimoto A, Ideta A, Aoyagi Y (2009). Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology 71: 68–73. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2008.09.016"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2008.09.016</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Hohenboken WD (1999). Application of sexed semen in cattle production. Theriogenology 52: 1421-1433. </span><a href="http://dx.doi.org/10.1016/S0093-691X(99)00227-7 "><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/S0093-691X(99)00227-7</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Hossein-Zadeh NG, Nejati-Javaremi A, Miraei-Ashtiani SR, Kohram H (2010). Bio-economic evaluation of the use of sexed semen at different conception rates and herd sizes in Holstein populations. Anim. Reprod. Sci. 121:17–23. </span><a href="http://dx.doi.org/10.1016/j.anireprosci.2010.05.012"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.anireprosci.2010.05.012</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Kasimanickam V, Kasimanickam R, Arangasamy A, Saberivand A, Stevenson JS, Kastelic JP (2012). Association between mRNA abundance of functional sperm function proteins and fertility of Holstein bulls. Theriogenology 78: 2007–2019. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2012.07.016"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2012.07.016</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Khalajzadeh S, Nejati-Javaremi A, Mehrbani Yeganeh HM (2012). Effect of widespread and limited use of sexed semen on genetic progress and reproductive performance of dairy cows. Animal 6-9: 1398–1406. </span><a href="http://dx.doi.org/10.1017/S1751731112000651"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1017/S1751731112000651</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Libbus BL, Perreault SD, Johnson LA, Pinkel D (1987). Incidence of chromosome aberrations in mammalian sperm stained with Hoechst 33342 and UV-laser irradiated during flow sorting. Mutat. Res.182: 265–274. </span><a href="http://dx.doi.org/10.1016/0165-1161(87)90011-2"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/0165-1161(87)90011-2</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">López-Gatius F, Santolaria P, Yániz J, Rutllant J, López-Béjar M (2002). Factors affecting pregnancy loss from gestation Day 38 to 90 in lactating dairy cows from a single herd. Theriogenology 57: 1251–1261. </span><a href="http://dx.doi.org/10.1016/S0093-691X(01)00715-4"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/S0093-691X(01)00715-4</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Lu Y, Zhang M, Lu S, Xu D, Huang W, Meng B, Xu H, Lu K (2010). Sex-preselected buffalo (Bubalus bubalis) calves derived from artificial insemination with sexed sperm. Anim. Reprod. Sci. 119:169–171. </span><a href="http://dx.doi.org/10.1016/j.anireprosci.2010.01.001"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.anireprosci.2010.01.001</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Pegorer MF, Vasconcelos JLM, Trinca LA, Hansen PJ, Barros CM (2007). Influence of sire and sire breed (Gyr versus Holstein) on establishment of pregnancy and embryonic loss in lactating Holstein cows during summer heat stress. Theriogenology 67: 692–697. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2006.09.042"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2006.09.042</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Pontes JH, Silva KC, Basso AC, Rigo AG, Ferreira CR, Santos GMG, Sanches BV, Porcionato JPF, Vieira PH, Faifer FS, Sterza FA, Schenk JL, Seneda MM (2010). Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus-taurus dairy cows using sexed sperm. Theriogenology 74: 1349–1355. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2010.06.004"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2010.06.004</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Rath D, Johnson LA (2008). Application and Commercialization of Flow Cytometrically Sex-Sorted Semen. Reprod. Dom. Anim. 43: 338–346. </span><a href="http://dx.doi.org/10.1111/j.1439-0531.2008.01182.x"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1111/j.1439-0531.2008.01182.x</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Sales JN, Crepaldi GA, Fosado M, Campos Filho EP, Baruselli PS (2010). Timing of insemination with sexed or nonsexed semen on pregnancy rates of Jersey heifers detected in heat by radiotelemetry. Reprod. Fertil. Develop. 22: 178–178. </span><a href="http://dx.doi.org/10.1071/RDv22n1Ab40"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1071/RDv22n1Ab40</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Schenk JL, Cran DG, Everett RW, Seidel GE Jr (2009). Pregnancy rates in heifers and cows with cryopreserved sexed sperm: Effects of sperm numbers per inseminate, sorting pressure and sperm storage before sorting. Theriogenology 71: 717–728. </span><a href="http://dx.doi.org/10.1016/j.theriogenology.2008.08.016"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/j.theriogenology.2008.08.016</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Seidel GE Jr (2003). Economics of selecting for sex: the most important genetic traits. Theriogenology 59: 585–598. </span><a href="http://dx.doi.org/10.1016/S0093-691X(02)01242-6"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/S0093-691X(02)01242-6</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Seidel GE Jr, Schenk JL, Herickhoff LA, Doyle SP, Brink Z, Green RD, Cran DG (1999). Insemination of heifers with sexed sperm. Theriogenology 52:1407–1420. </span><a href="http://dx.doi.org/10.1016/S0093-691X(99)00226-5"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1016/S0093-691X(99)00226-5</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Sørensen MK, Voergaard J, Pedersen LD, Berg P, Sørensen AC (2011). Genetic gain in dairy cattle populations is increased using sexed semen in commercial herds. J. Anim. Breed. Genet. 128: 267–275. </span><a href="http://dx.doi.org/10.1111/j.1439-0388.2011.00924.x"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.1111/j.1439-0388.2011.00924.x</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">VanRaden PM, Miller RH (2006). Effects of nonadditive genetic interactions, inbreeding and recessive defects on embryo and fetal loss by seventy days. J Dairy Sci 89, 2716–2721. </span><a href="http://dx.doi.org/10.3168/jds.S0022-0302(06)72347-5"><span class="Hyperlink CharOverride-45">http://dx.doi.org/10.3168/jds.S0022-0302(06)72347-5</span></a></li>
                <p>&nbsp;</p>
				<li class="References ParaOverride-8" lang="en-US"><span class="CharOverride-45">Weigel KA (2004). Exploring the role of sexed semen in dairy production systems. J. Dairy Sci. 87 (E. Suppl.): E120–E130. </span></li>
                <p>&nbsp;</p>
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