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<h1 class="Hdr1">

Research Article


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Advances in Animal and Veterinary Sciences 2 (6): 359 – 364


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<div>
<h2 class="Hdr2">

Effect of Weaning Age and Sex on Meat Quality Traits of Pigs Raised under Intensive System and Slaughtered at 70 Kg Body Weight

</h2>

<p class="p3">

<strong>Batlhalefi Ganelang</strong><span><sup>1*</sup></span>, 

<strong>John Cassius Moreki</strong><span><sup>1</sup></span>, 

<strong>Shalaulani James Nsoso</strong><span><sup>1</sup></span>, 

<strong>Christopher Mareledi Tsopito</strong><span><sup>1</sup></span>
</p>


</div>

<div class="p4">

<ul>

<li>Department of Animal Science and Production, Botswana College of Agriculture, Private Bag 0027, Gaborone, Botswana.</li>

</ul>

</div><div>        
  <p><span class="Author">*Corresponding author: bganelang@live.com</span></p>

</div>
<div id="ColorID"><p>
<span><b>ARTICLE CITATION:</b></span> 

Ganelang B, Moreki JC, Nsoso SJ, Tsopito CM (2014). Effect of weaning age and sex on meat quality traits of pigs raised under intensive system and slaughtered at 70 kg body weight. Adv. Anim. Vet. Sci. 2 (6): 359 – 364.

<br>
<span><b>Received: </b></span>2013–06–10, <span><b>Revised: </b></span>2014–07–03, <span><b>Accepted: </b></span>2014–07–04<br>

The electronic version of this article is the complete one and can be found online at 

(<a href="http://dx.doi.org/10.14737/journal.aavs/2014/2.6.359.364">

http://dx.doi.org/10.14737/journal.aavs/2014/2.6.359.364

</a>)
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited </p>
</div>

<div>
     <h3 class="Hdr3">ABSTRACT</h3>

    <p>
The aim of the study was to determine the effects of weaning age and sex on carcass quality of pigs slaughtered at 70 kg body weight. A total of 24 piglets were purchased at birth from a commercial farmer and were assigned to three weaning ages: 21 days (treatment 1), 28 days (treatment 2) and 35 days (treatment 3). Feed and water were given ad libitum up to 70 kg slaughter weight. Pigs were slaughtered humanely using electrical stunning and the carcasses were dissected into two halves, right and left. From the left carcass, four 2.5 cm thick chops were removed from longissimus muscle. The chops were cut at the 9th, 12th, 13th and 14th ribs to measure tenderness, pH, drip loss and meat colour, respectively. Meat pH was measured using pH meter with glass probe while drip loss was measured using gravimetric method. Meat colour was measured on chops taken at 13th rib using Konica Minolta Chroma Meter CR-410 (Konica Minolta Sensing, INC., Japan) while tenderness was measured on boiled meat using TMS light weight blade (1.2 mm thick) attached to a 2.5 KN TMS PRO texture analyser (Food Technology Corporation, West Sussex, UK). Data were analysed as completely randomised design (CRD) using the General Linear Model (GLM) of statistical analysis system, version 9.3. Weaning age did not have significant (P > 0.05) effect on meat quality. Sex had significant (P = 0.03) effect on yellowness of meat of pigs weaned at 35 days of age. Males had significantly more yellow meat (9.50 ± 0.45) than females (7.63 ± 0.45). However, sex had no significant effect on drip loss, lightness, redness, pH and tenderness. Piglets can be weaned at 21, 28 or 35 days of age without negatively affecting meat quality. A 21 day weaning age is recommended as pigs weaned at 21 days reach market weight early.    </p>
 
</div>




<div>
  
  <h3 class="Hdr3">INTRODUCTION</h3>
    <p>
    
  Weaning age is an extremely important management decision in commercial pig production, which is based upon many factors including sow performance, herd health, pig performance, costs and revenues associated with these factors such as lactation space utilization, weaned pig value (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Smith 2006" target="_self">Smith et al., 2006</a>). Weaning is usually undertaken in one of the following four categories: conventional weaning (3–5 weeks of age); early weaning (10 days of age to 3 weeks); segregated early weaning (less than 18 days) and medicated early weaning (5 days) (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Roese 2006" target="_self">Roese and Taylor, 2006</a>). The age at which piglets are separated from the sow has been identified as an animal welfare risk in current pig production systems (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Whiting 2008" target="_self">Whiting and Pasma, 2008</a>). Weaning results in several environmental and social stresses on the piglet as it adapts to a new environment and feed source (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Collins 2010" target="_self">Collins et al., 2010</a>). <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Johnson 2012" target="_self">Johnson et al. (2012)</a> stated that farm economics and productivity, sow and piglet welfare, and available facilities will dictate the best weaning age on a given farm.<br>
Meat colour, pH, tenderness and drip loss are some of the major meat quality traits in pork. The amount of water lost when pork is cut is called “drip loss”. Drip loss often increases when pork is pale or pH is low. Lower drip loss values indicate lower loss of water which is preferable to consumers (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Lammers 2012" target="_self">Lammers et al., 2012</a>). Drip loss affects appearance, nutritional value and palatability of the meat to the consumer (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Down S (2013)" target="_self">Down, 2013</a>). Meat colour is a major determinant of appearance. Appearance is practically the only criterion the consumer can use to judge the acceptability of most meats at purchase (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Lammers 2012" target="_self">Lammers et al., 2012</a>). The difference in the pH of meat is due to the amount of glycogen present in the muscle at slaughter (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Lonergan, S. (2012)" target="_self">Lonergan, 2012</a>), which is directly proportional to the potential amount of lactic acid. A higher pH indicates a lower drip loss and better eating pork (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Lammers 2012" target="_self">Lammers et al., 2012</a>). The main factors affecting meat tenderness are connective tissue, intramuscular fat content, and myofibrillar structure. Tenderness is associated with size of the muscle fibres. The smaller the fibres the more tender the meat. Tenderness decreases with age (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Cunningham 2005" target="_self">Cunningham et al., 2005</a>).<br>
There is still much debate about what age piglets should be weaned (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Shipka 2011" target="_self">Shipka, 2011</a>; <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#King G (2013)" target="_self">King, 2013</a>). Therefore, it is important to identify age at weaning suitable for Botswana conditions that would improve meat quality of pigs. This study was carried out to determine the effects of weaning age and sex on meat quality of pigs raised under intensive system and slaughtered at 70 kg body weight. </p>
  
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<div>
<h3 class="Hdr3">MATERIALS AND METHODS</h3>
<p>

The experiment was carried out at Chemae Farm, near Matebeleng village in Kgatleng District from May to November 2013. The site is situated on 24o 32΄ 54.12΄΄S 26o 1΄ 3.55΄΄E, at an altitude of 980 m above sea level. The area has average daily temperature of 15 oC in winter (May to July) and 29 oC in summer (August to November).
</p><p><strong>Animal management</strong><br>
Forty eight piglets [(Landrace x Large white) x Topigs (Tempo x Topigs 40) x Topigs cross] were randomly assigned to three treatments. Treatment 1 was for piglets weaned at 21 days, treatment 2 (28 days) and treatment 3 (35 days). A 28 day weaning period served as a control. There were 4 replicates in each treatment and each replicate had 4 pigs (2 males and 2 females). Teeth and tail cutting were performed when piglets were one day old while iron injection and castration were performed at three days of age. Pigs were not vaccinated against any disease but were treated for internal parasites using piperazine (active ingredient: piperazine adipate 100%) at 1.2 g/10 kg body weight. Triatix pour on dip (active ingredient: amitraz 2% m/v) was used to treat external parasites. Cosumix Plus (active ingredients: sulphachlorpyridazine sodium and trimethoprim) and sulfazine 33 1/3 % (active ingredient: sulphadimidine) were used to treat pigs for diarrhoea. Piglets were raised in 12 pens each measuring 2.2 m x 2.2 m in a naturally ventilated house with concrete floor and corrugated iron roofing. Wheat straw was used as bedding material and was replaced every fortnight. During cold days heating was provided to the piglets using coal.</p><p><strong>
Diets</strong><br>
The pigs in all treatments were fed commercial pig diets that complied with Botswana Bureau of Standards pig feeds specification (BOS 190:2006). From 10 to 35 days of age, piglets were offered creep diet, weaner diet from 35 to 70 days of age and thereafter grower diet up to 70 kg slaughter weight. Feed and water were provided ad libitum throughout the study period. The analysed nutritive composition of the diets expressed in g/kg is shown in Table 1 </p>

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<div class="pt" > <a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table1_464.png" target="new"><img class="img_display" src="http://nexusacademicpublishers.com/uploads/imagesfiles/Table1_464.png" width="80" height="80"></a>
<p class="Txt_Dis" ><span><b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table1_464.png" target="new">Table 1: </a></b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table1_464.png"><span>Nutrient composition of diets offered to pigs of pigs raised under intensive system</a></p>

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<div><p>
<strong>Ante mortem treatment</strong><br>  
At 70 kg body weight, pigs were transported early in the morning (i.e., 0700 hours) in an open vehicle with rails to local abattoir in Gaborone for slaughter. Upon arrival at the abattoir, pigs were held in lairage for 12 hours with free access to water until slaughter the following morning. The animals were slaughtered humanely by electrical stunning (240 V, 0.5 A, 5s), exsanguinated, scalded, dehaired and eviscerated (Serrano et al., 2009). After slaughter, the carcasses were hanged to allow blood to drain away. Ante mortem and post-mortem inspections were carried out by a meat inspector as per Botswana Livestock and Meat Industries Act of 2007. After inspection, the carcasses were chilled at 7 oC for 24 hours.</p><p>
<strong>
Data Collection</strong><br>
The carcasses were dissected into two halves, right and left. From the left carcass, four 2.5 cm thick chops were removed from longissimus muscle (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Corino 2008" target="_self">Corino et al., 2008</a>). The chops were cut at the 9th, 12th, 13th and 14th ribs to measure tenderness, pH, drip loss and meat colour, respectively. Meat pH was measured using pH meter with glass probe (CRISON pH meter, model GLP 21). The probe was inserted into a cut made at the centre of the loin to take a reading. Before taking the reading, the probe was rinsed in distilled water.
Pork chops of 2.5 cm thickness weighing 30 g (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Honikel KO (1998)" target="_self">Honikel, 1998</a>) from longissimus muscle were cut and trimmed of fat and connective tissues (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Milligan 1998" target="_self">Milligan  et al., 1998</a>) to measure drip loss. Each sample was placed in a plastic net and suspended in a box wrapped with aluminium foil to prevent entry of air. The samples were suspended such that they did not touch the surface of the box. The samples were stored at 4 oC for 24 hours and thereafter removed from the net, blotted with tissue paper to remove water on their surfaces and individually reweighed. The samples were evaluated in duplicate and the average of the two was calculated and used as measure of the sample drip loss. The difference between initial weight and final weight was considered as drip loss. The formula (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Honikel KO (1998)" target="_self">Honikel, 1998</a>) used for calculating percentage drip loss was;<br>
% drip loss = (initial weight – final weight)/initial weight x 100
Meat colour was measured on chops taken at 13th rib using Konica Minolta Chroma Meter CR-410 (Konica Minolta Sensing, INC., Japan), D65 illuminant calibrated against a white calibration tile (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Milligan 1998" target="_self">Milligan et al., 1998</a>). The colour difference method was employed using the white calibration tile as the colour difference target to give colour change with the meat colour readings. The L*a*b system was used to measure colour. The parameters L* (lightness), a* (redness) and b* (yellowness), were measured on each pork chop surface.
Peak shear force (which was used as measure of meat tenderness) was measured on cooked meat samples. A fresh 2.5 cm-thick longissimus muscle slice was boiled in a pressure cooker for 5 minutes and left to cool for 10 minutes and blotted dry. After cooking, three 1.0 cm thick and 3.0 cm long slices were cut once perpendicular to the fibre orientation, with TMS light weight blade (1.2 mm thick) attached to a 2.5 KN TMS PRO texture analyser (Food Technology Corporation, West Sussex, UK). </p><p>
<strong>
Statistical Analysis</strong><br>
Data were analysed as completely randomised design (CRD) with treatments arranged factorially using the General Linear Model (GLM) procedure of Statistical Analysis System, version 9.3 (SAS Institute, 2010). Means were separated using least significant difference (t test). The significance level considered for all the statistical tests was P < 0.05. The following statistical model was used for analysis;
<br>
Yijk = µ + Wi + Sj + (WS)ij + β(AGEijk – AGE) + εijk
where Yijk is the response variable (carcass quality: meat pH, drip loss, meat colour, meat tenderness) 
µ is the general mean, 
Wi is the effect of weaning age at level i (21 days, 28 days, 35 days), 
Sj is the effect of sex at level j (male, female), 
(WS)ij is the effect of interaction WS at level ij, 
β is linear regression coefficient of AGEijk on age at slaughter,
AGE is the mean age of animals at slaughter,
AGEijk is the age of individual animals at slaughter,
Eijk is the random error.



</p></div>


<div class="pt" > <a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table2_464.png" target="new"><img class="img_display" src="http://nexusacademicpublishers.com/uploads/imagesfiles/Table2_464.png" width="80" height="80"></a>
<p class="Txt_Dis" ><span><b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table2_464.png" target="new">Table 2: </a></b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table2_464.png"><span>Means and standard errors of drip loss, pH, shear force and colour of meat of pigs raised under intensive system, weaned at different ages and slaughtered at 70 kg body weight</a></p>

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<div><h3 class="Hdr3">RESULTS AND DISCUSSION</h3><p>
Weaning age did not have significant (P = 0.15) effect on meat pH of pigs slaughtered at 70 kg body weight (Table 2). However, pigs weaned at 35 days of age tended to have higher pH (5.85) than those weaned at 21 days (5.77) and 28 days (5.78). The current results are in agreement with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Jiang 2012" target="_self">Jiang et al. (2012) </a>and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Bérard 2008" target="_self">Bérard et al. (2008)</a> who reported pH values of 5.63 - 5.92 and 5.6 - 5.7, respectively for pigs weaned at 35 days of age. In agreement with the current results, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Beaulieu 2010" target="_self">Beaulieu et al. (2010)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Gentry 2004" target="_self">Gentry et al. (2004)</a> also reported pH values of 5.69 and 5.60, respectively for pigs weaned at 21 days of age. However, the current results are lower than the pH value of 5.92 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Morcuende 2007" target="_self">Morcuende (2007)</a> for pigs weaned at 21 days of age. <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Kuhn 2004" target="_self">Kuhn et al. (2004)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Millet  2004" target="_self">Millet et al. (2004)</a> reported pH of 5.46 and 5.45, respectively, for piglets weaned at 28 days which is lower than the value of 5.78 found in the current study. A slightly higher pH of 5.71 for pigs weaned at 28 days was reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Oksbjerg  2002" target="_self">Oksbjerg et al. (2002)</a>. Weaning age did not have a significant (P = 0.18  ) effect on drip loss of chops from pigs slaughtered at 70 kg body weight (Table 2 ). However, pigs weaned at 35 days of age tended to have higher drip loss (5.50%) than those weaned at 21 days (4.81%) and 28 days (5.35%). The current results are in agreement with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Bérard 2008" target="_self">Bérard et al. (2008)</a> who reported a drip loss of 5.2 to 6.1% for pigs weaned at 35 days of age. <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Jiang 2012" target="_self">Jiang et al. (2012)</a> reported lower drip loss of 2.16 - 2.83% for pigs weaned at the same age. Drip loss for pigs weaned at 28 days of age was higher (5.35%) in the current study compared to a drip loss of 3.66% reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Alexander 2008" target="_self">Alexander et al. (2008)</a>. Compared to the current result (5.35 %), <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Oksbjerg  2002" target="_self">Oksbjerg et al. (2002)</a> reported 9.1% drip loss for pigs weaned at 28 days of age. The differences in drip loss values of the current study and previous studies could be due to differences in ante mortem conditions. 
Weaning age did not have a significant (P = 0.77  ) effect on meat tenderness of pigs slaughtered at 70 kg body weight (Table 2 ). Weaning pigs at 21 days of age resulted in tougher meat (112.21 N) than 28 days (111.43 N) and 35 days (109.85 N) weaning ages. Pigs weaned at 35 days tended to produce the softest meat. These values are higher than the shear force values of 44 to 45 N reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Bérard 2008" target="_self">Bérard et al. (2008)</a> for pigs weaned at 35 days. The difference in shear force values in the present study compared to <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Bérard 2008" target="_self">Bérard et al. (2008)</a> study could be due to differences in the cooking methods and blades used. In the current study, TMS light weight blade (1.2 mm thick) was used on boiled meat while in the study by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Bérard 2008" target="_self">Bérard et al. (2008)</a>, Warner-Bratzler shear blade (2.5-mm-thick) was used on grilled meat. <br>
Weaning age did not have significant  (P > 0.05) (effect on L (measure of lightness), a (measure of redness) and b (measure of yellowness) values of colour. Pigs weaned at 21 days of age tended to have lighter meat (48.60) than those weaned at 28 days (47.32) and 35 days (46.23). Furthermore, pigs weaned at 21 days of age tended to have redder meat (15.83) than those weaned at 28 days (14.22) and 35 days (13.65). Pigs weaned at 28 days produced meat that was more yellow (9.03) than those weaned at 35 days (8.56). The L values found in the current study falls within the reddish-pink, firm and non-exudative (RFN) range of 43-49 (American Meat Science Association, 2001). The current results are in agreement with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Morcuende 2007" target="_self">Morcuende et al. (2007)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Gentry 2004" target="_self">Gentry et al. (2004)</a> who reported Minolta L values of 47.84 and 47.9, respectively for pigs weaned at 21 days of age. <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Beaulieu 2010" target="_self">Beaulieu et al. (2010)</a> reported slightly higher Minolta L value of 50.45 for pigs weaned at 21 days. The L value of 47.32 for pigs weaned at 28 days reported in the current study is consistent with 48.28 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Kuhn 2004" target="_self">Kuhn et al. (2004)</a> but lower than 55.9 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Millet  2004" target="_self">Millet et al. (2005)</a> for pigs weaned at the same age.<br>
The ‘a’ value of 15.83 for pigs weaned at 21 days reported in the current study is higher than the values of 9.56 and 3.00 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Morcuende 2007" target="_self">Morcuende et al. (2007)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Gentry 2004" target="_self">Gentry et al. (2004)</a>, respectively for pigs weaned at the same age (21 days). In the current study, pigs weaned at 28 days had “a” value of 14.22 which is higher than the 8.84 and 7.14 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Kuhn 2004" target="_self">Kuhn et al. (2004)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Millet  2004" target="_self">Millet et al. (2005)</a>, respectively for pigs weaned at 28 days.<br> 
The ‘b’ value of 9.02 for pigs weaned at 21 days reported in the current study is higher than the value of 4.88 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Morcuende 2007" target="_self">Morcuende et al. (2007)</a> but lower than 12.00 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Gentry 2004" target="_self">Gentry et al. (2004)</a> for pigs weaned at 21 days. Furthermore, the ‘b’ value of 9.03 for pigs weaned at 28 days in the current study is lower than 16.66 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Millet  2004" target="_self">Millet et al. (2005) </a>but higher than -0.23 reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Kuhn 2004" target="_self">Kuhn et al. (2004)</a> for pigs weaned at the same age. <br>
Slaughter age for pigs weaned at 21 and 35 days was significantly (P = 0.04  ) different (Table 2 ). Pigs weaned at 35 days of age took a longer time (137 days) to reach market weight (70 kg) compared to those weaned at 21 days (130.8 days) and 28 days (134.6 days). However, there was no significant difference in slaughter age between 28 day weaning age and other weaning ages. These results disagree with Dritz et al. (1996) who found no significant difference in slaughter age between 9 days and 19 days weaning ages for pigs slaughtered at 109 kg. 



</p></div>

<div class="pt" > <a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table3_464.png" target="new"><img class="img_display" src="http://nexusacademicpublishers.com/uploads/imagesfiles/Table3_464.png" width="80" height="80"></a>
<p class="Txt_Dis" ><span><b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table3_464.png" target="new">Table 3: </a></b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table3_464.png"><span>Means and standard errors of drip loss, pH, shear force and colour of meat of pigs raised under intensive system and weaned at 21 days of age</a></p>

</div>

<div><p>
Sex had no significant (P = 0.75 ) effect on pH of meat of pigs weaned at 21, 28 and 35 days (Tables 3 to 5 ). However, males tended to have higher pH values than females in all the weaning ages. The current results agree with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Piao 2004" target="_self">Piao  et al. (2004)</a>, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Correa 2006" target="_self">Correa et al. (2006)</a>, and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Đurkin 2012" target="_self">Đurkin et al. (2012) </a>who observed no significant difference between gilts and barrows for the pH of longissimus muscle. However, the current results disagree with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#D'Souza 2002" target="_self">D’Souza and Mullan (2002)</a> who found significantly lower ultimate pH in gilts (5.46) than in barrows (5.73). </p></div>


<div class="pt" > <a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table4_464.png" target="new"><img class="img_display" src="http://nexusacademicpublishers.com/uploads/imagesfiles/Table4_464.png" width="80" height="80"></a>
<p class="Txt_Dis" ><span><b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table4_464.png" target="new">Table 4: </a></b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table4_464.png"><span>Means and standard errors of drip loss, pH, shear force and colour of meat of pigs raised under intensive system and weaned at 28 days of age</a></p>

</div>


<div><p>
Sex had no significant (P ) effect on drip loss of meat from pigs weaned at 21, 28 and 35 days (Tables 3  to 5 ). However drip loss tended to be higher in females than male pigs weaned at 28 and 35 days of age. Male pigs weaned at 21 days tended to have higher drip loss (5.17%) than females (4.46%). Drip loss reported in the current study is lower compared to the values of 8.6% for barrows and 9.5% for gilts reported by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Jaturasitha 2006" target="_self">Jaturasitha et al. (2006)</a>. The differences in drip loss could be due to the extent to which glycogen was degraded in the muscle which affect lactic acid concentration. High concentration of lactic acid leads to high drip loss. Similarly, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Đurkin 2012" target="_self">Đurkin et al. (2012)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Correa 2006" target="_self">Correa et al. (2006)</a> observed no significant difference between gilts and barrows for drip loss. On the other hand, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Piao 2004" target="_self">Piao et al. (2004)</a> observed higher drip loss in barrows than in gilts. <br>
Sex had no significant (P = 0.67  ) effect on meat tenderness of pigs weaned at 21, 28 and 35 days (Tables 3 to 5 ). However, meat from male pigs tended to be more tender than that of females. The current results agree with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Latorre 2004" target="_self">Latorre et al. (2004)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Đurkin 2012" target="_self">Đurkin et al. (2012)</a> who found no significant differences between barrows and gilts in meat tenderness. However, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Jaturasitha 2006" target="_self">Jaturasitha et al. (2006)</a> found significant difference in shear force between barrows (26.5 N) and gilts (33.9 N). This difference in results could be due to more intramuscular fat found in barrows  than in gilts (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Latorre 2003" target="_self">Latorre et al., 2003</a>). </p></div>



<div class="pt" > <a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table5_464.png" target="new"><img class="img_display" src="http://nexusacademicpublishers.com/uploads/imagesfiles/Table5_464.png" width="80" height="80"></a>
<p class="Txt_Dis" ><span><b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table5_464.png" target="new">Table 5: </a></b><a href="http://nexusacademicpublishers.com/uploads/imagesfiles/Table5_464.png"><span>Means and standard errors of drip loss, pH, shear force and colour of meat of pigs raised under intensive system and weaned at 35 days of age</a></p>

</div>

<div><p>
Sex had significant (P = 0.03 ) effect on yellowness of meat from pigs weaned at 35 days of age but not in other weaning ages (Tables 3 to 5 ). Males had significantly more yellow meat (9.50) than females (7.63). However, sex had no significant difference in brightness and redness of meat produced by pigs irrespective of age at weaning. For pigs weaned at 21 days, males tended to produce brighter meat (48.71) than females (48.49). Males tended to produce redder meat than females in all the weaning ages. The current results agree with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Cisneros 1996" target="_self">Cisneros et al. (1996)</a> who found that meat from barrows have a higher myoglobin content than meat from gilts. In this study, males tended to produce meat that was more yellow than females in pigs weaned at 28 and 35 days of age. Previous studies by <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Latorre 2004" target="_self">Latorre et al. (2004)</a>, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Đurkin 2012" target="_self">Đurkin et al. (2012)</a> and (<a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Correa 2006" target="_self">Correa et al., 2006</a>) found that sex had no effect on meat colour. On the contrary, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Alonso 2009" target="_self">Alonso et al. (2009)</a> reported more intense meat colour in males than females. <br>
Sex did not significantly (P >0.05) influence slaughter age (Tables 3 to 5 ). However, females tended to take longer to reach 70 kg slaughter weight compared to males. <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Latorre 2003" target="_self">Latorre et al. (2003)</a>, <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Morales 2011" target="_self">Morales et al. (2011)</a> and <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Piao 2004" target="_self">Piao et al. (2004)</a> argued that males eat more feed and hence grow faster than their female counterparts. The current findings are in disagreement with <a href="http://www.nexusacademicpublishers.com/table_contents_detail/4/341/html/#Serrano 2009" target="_self">Serrano et al. (2009)</a> who found females to be superior in growth performance to their castrated male counterparts. </p><div><h3 class="Hdr3">CONCLUSION</h3><p>

Drip loss, pH, tenderness, colour (lightness and redness) and tenderness of meat from pigs raised under intensive system were not affected by weaning age and sex. Weaning at 35 days of age promoted production of yellow meat. These piglets can be weaned at 21, 28 or 35 days of age under intensive system without negatively affecting meat quality. A 21 day weaning age is recommended as piglets weaned at 21 days of age reach slaughter weight earlier than other weaning ages.
	</p><div><h3 class="Hdr3">ACKNOWLEDGEMENT</h3><p>
	
The authors would like to thank the sponsors of the research, Botswana government for the financial assistance needed to carry out the study. Management of Chemae farm are thanked for providing the facilities for carrying out the trials. The following institutions are thanked for helping in analyzing the samples and data; University of Botswana, Ministry of Agriculture (Botswana) and Botswana National Food Research and Technology Centre.

</p></div>

<div>
<h3 class="Hdr3">REFERENCES</h3>
 
<p class="p2"> <a name="Alexander 2008"></a>
Alexander LS, Qu A, Cutler SA, Mahajan A, Lonergan SM, Rothschild MF, Weber TE, Kerr BJ, Stahl CH  (2008). Response to dietary phosphorus deficiency is affected by genetic background in growing pigs. J. Anim. Sci. 86: 2585 – 2595.<br /><a href="http://dx.doi.org/10.2527/jas.2007-0692" target="_blank">http://dx.doi.org/10.2527/jas.2007-0692</a><br />PMid:18502882                                                          

</p><p class="p2"> <a name="Alonso 2009"></a>Alonso V, Campo M, Del M, Espa-ol S, Roncalés P, Beltrán JA (2009). Effect of crossbreeding and gender on meat quality and fatty acid composition in pork. Meat sci. 81(1): 209 - 217.<br /><a href="http://dx.doi.org/10.1016/j.meatsci.2008.07.021" target="_blank">http://dx.doi.org/10.1016/j.meatsci.2008.07.021</a><br />PMid:22063984                                                          

</p><p class="p2"> <a name="American 2001"></a>American Meat Science Association (2001). Meat evaluation handbook. Am. Meat Sci. Assoc. (AMSA). Savoy, USA. pp 86 - 110.                                                      

</p><p class="p2"> <a name="Beaulieu 2010"></a>Beaulieu AD, Aalhus JL, Williams NH, Patience JF (2010). Impact of piglet birth weight, birth order, and litter size on subsequent growth performance, carcass quality, muscle composition, and eating quality of pork. J. Anim. Sci. 88: 2767 – 2778.<br /><a href="http://dx.doi.org/10.2527/jas.2009-2222" target="_blank">http://dx.doi.org/10.2527/jas.2009-2222</a><br />PMid:20418451                                                          


</p><p class="p2"> <a name="Bérard 2008"></a>Bérard J, Kreuzer M, Bee G (2008). Effect of litter size and birth weight on growth, carcass and pork quality, and their relationship to postmortem proteolysis. J. Anim. Sci. 86:2357 – 2368.<br /><a href="http://dx.doi.org/10.2527/jas.2008-0893" target="_blank">http://dx.doi.org/10.2527/jas.2008-0893</a><br />PMid:18469061                                                          

</p><p class="p2"> <a name="Botswana 2007"></a>Botswana Livestock and Meat Industries Act of 2007.                                                      


</p><p class="p2"> <a name="Collins 2010"></a>Collins CL, Leury BJ, Dunshea FR (2010). Early weaning has minimal effects on lifetime growth performance and body composition of pigs. Ani. Prod. Sci. 50: 79 – 87.<br /><a href="http://dx.doi.org/10.1071/AN09059" target="_blank">http://dx.doi.org/10.1071/AN09059</a>                                                      

</p><p class="p2"> <a name="Cisneros 1996"></a>Cisneros F, Ellis M, Mckeith FK, McCaw J, Fernando RL (1996). Influence of slaughter weight on growth and carcass characteristics, commercial cutting and curing yields, and meat quality of barrows and gilts from two genotypes. J. Anim. Sci. 74: 925 - 933.<br />PMid:8726722                                                          
</p><p class="p2"> <a name="Corino 2008"></a>Corino C, Musella M, Pastorelli G, Rossi R, Paolone K, Costanza L, Manchisi A, Maiorano G (2008). Influences of dietary conjugated linoleic acid (CLA) and total lysine content on growth, carcass characteristics and meat quality of heavy pigs. Meat Sci. 79:  307 – 316.<br /><a href="http://dx.doi.org/10.1016/j.meatsci.2007.10.001" target="_blank">http://dx.doi.org/10.1016/j.meatsci.2007.10.001</a><br />PMid:22062759                                                          


</p><p class="p2"> <a name="Correa 2006"></a>Correa JA, Faucitano L, Laforest JP, Rivest J, Marcoux M, Garie C (2006). Effects of slaughter weight on carcass composition and meat quality in pigs of two different growth rates. Meat Sci. 72: 91 – 99.<br /><a href="http://dx.doi.org/10.1016/j.meatsci.2005.06.006" target="_blank">http://dx.doi.org/10.1016/j.meatsci.2005.06.006</a><br />PMid:22061378                                                         

 </p><p class="p2"> <a name="Cunningham 2005"></a>Cunningham M, Latour MAM, Acker D (2005). Animal Science and Industry (Seventh Edition). Pearson Prentice Hall. New Jersey, USA. Pp: 516 - 518.                                                      

</p><p class="p2"> <a name="Dritz 1996"></a>Dritz SS, Owen KQ, Nelssen JL, Goodband RD, Tokach MD (1996). Effect of weaning age and nursery diet complexity on growth performance and carcass characteristics and composition of high health status pigs from weaning to 109 kilograms. J. Anim. Sci. 74: 2975 - 2984.<br />PMid:8994912                                                          

</p><p class="p2"> <a name="Down S (2013)"></a>Down S (2013). Pork processing: Proteins influencing water holding capacity and drip loss. Retrieved 22/10/2013 from http://www.spectroscopynow.com/details/ezine/13e5bb0ddb1/Pork-processing-Proteins-influencing-water-holding-capacity-and-drip-loss.html?tzcheck=1                                                      

</p><p class="p2"> <a name="D'Souza 2002"></a>D'Souza DN, Mullan BP (2002). The effect of genotype, sex and management strategy on the eating quality of pork. Meat Sci. 60: 95 –101.<br /><a href="http://dx.doi.org/10.1016/S0309-1740(01)00112-7" target="_blank">http://dx.doi.org/10.1016/S0309-1740(01)00112-7</a>                                                      

</p><p class="p2"> <a name="Đurkin 2012"></a>Đurkin I, Dadić M,  Brkić D, Lukić B, Kušec G, Mikolin M, Jerković I (2012). Influence of gender and slaughter weight on meat quality traits of heavy pigs. Retrieved 06/11/2013 from http://aas.bf.uni-lj.si/zootehnika/supl/3-2012/PDF/3-2012-211-214.pdf                                                      

</p><p class="p2"> <a name="Gentry 2004"></a>Gentry JG, McGlone JJ, Miller MF, Blanton Jr. JR (2004). Environmental effects on pig performance, meat quality, and muscle characteristics. J. Anim. Sci. 82: 209 – 217.<br />PMid:14753363                                                          

</p><p class="p2"> <a name="Honikel KO (1998)"></a>Honikel KO (1998). Reference methods for the assessment of physical characteristics of meat. Meat Sci. 49(4): 447 - 457.<br /><a href="http://dx.doi.org/10.1016/S0309-1740(98)00034-5" target="_blank">http://dx.doi.org/10.1016/S0309-1740(98)00034-5</a>                                                      


</p><p class="p2"> <a name="Jaturasitha 2006"></a>Jaturasitha S, Kamopas S, Suppadit T, Khiaosa-arda R, Kreuzer M (2006). The effect of gender of finishing pigs slaughtered at 110 kilograms on performance, carcass and meat quality. Sci. Asia. 32: 297 - 305.<br /><a href="http://dx.doi.org/10.2306/scienceasia1513-1874.2006.32.297" target="_blank">http://dx.doi.org/10.2306/scienceasia1513-1874.2006.32.297</a>                                                      

</p><p class="p2"> <a name="Jiang 2012"></a>Jiang YZ, Zhu L, Tang GQ, Li MZ, Jiang AA, Cen WM, Xing SH,  Chen JN, Wen AX, He T, Wang Q, Zhu GX, Xie M, Li XW (2012). Carcass and meat quality traits of four commercial pig crossbreeds in China. Genetics and Molecular Research. Retrieved 24/10/2013 from http://www.funpecrp.com.br/gmr/year2012/vol11-AOP/pdf/gmr2050.pdf<br /><a href="http://dx.doi.org/10.4238/2012.September.19.6" target="_blank">http://dx.doi.org/10.4238/2012.September.19.6</a><br /><a href="http://dx.doi.org/10.4238/2012.January.27.3" target="_blank">http://dx.doi.org/10.4238/2012.January.27.3</a>

</p><p class="p2"> <a name="Johnson 2012"></a>Johnson AK, McGlone JJ, Gentry-Carter JG (2012). How does weaning age affect the welfare of the nursery pig?. Retrieved 14/08/13 from http://www.porkgateway.org/FileLibrary/PIGLibrary/Factsheets/a6764v1-0.pdf

</p><p class="p2"> <a name="King G (2013)"></a>King G (2013). Considerations at weaning time. Retrieved 13/08/13 from http://www.aps.uoguelph.ca/~gking/Ag_2350/wean.htm                                                      

</p><p class="p2"> <a name="Kuhn 2004"></a>Kuhn G, Kanitz E, Tuchscherer M, Nu¨rnberg G, Hartung M, Ender K, Rehfeldt C (2004). Growth and carcass quality of offspring in response to porcine somatotropin (pST) treatment of sows during early pregnancy. Livestock Prod. Sci. 85: 103 – 112.<br /><a href="http://dx.doi.org/10.1016/S0301-6226(03)00130-1" target="_blank">http://dx.doi.org/10.1016/S0301-6226(03)00130-1</a>                                                      

</p><p class="p2"> <a name="Lammers 2012"></a>Lammers PJ, Stender DR, Honeyman MS (2012). Pork quality. Retrieved 22/10/2013 from http://www.ipic.iastate.edu/publications/610.PorkQuality.pdf                                                      

</p><p class="p2"> <a name="Latorre 2003"></a>Latorre MA, Lazaro R, Gracia MI, Nieto M, Mateos GG (2003). Effect of sex and terminal sire genotype on performance, carcass characteristics, and meat quality of pigs slaughtered at 117 kg body weight. Meat Sci. 65: 1369 – 1377.<br /><a href="http://dx.doi.org/10.1016/S0309-1740(03)00059-7" target="_blank">http://dx.doi.org/10.1016/S0309-1740(03)00059-7</a>                                                      

</p><p class="p2"> <a name="Latorre 2004"></a>Latorre, M. A., Lazaro, R., Valencia, D. G., Medel, P. and Mateos, G. G. (2004). The effects of gender and slaughter weight on the growth performance, carcass traits, and meat quality characteristics of heavy pigs. Journal of Animal Science, 82: 526–533.<br />PMid:14974552                                                          

</p><p class="p2"> <a name="Lonergan, S. (2012)"></a>Lonergan, S. (2012). Pork quality: pH decline and pork quality. Retrieved 22/10/2013 from http://www.porkgateway.org/FileLibrary/PIGLibrary/Factsheets/a6647v1-0.pdf                                                      

</p><p class="p2"> <a name="Millet  2004"></a>Millet S, Raes K, Van den Broeck W, De Smet S, Janssens GPJ (2004). Performance and meat quality of organically versus conventionally fed and housed pigs from weaning till slaughtering. Meat Sci., 69: 335 – 341.<br /><a href="http://dx.doi.org/10.1016/j.meatsci.2004.08.003" target="_blank">http://dx.doi.org/10.1016/j.meatsci.2004.08.003</a><br />PMid:22062826                                                         

</p><p class="p2"> <a name="Milligan 1998"></a>Milligan SD, Ramsey CB, Miller MF, Kaster CS, Thompson LD (1998). Resting of pigs and hot-fat trimming and accelerated chilling of carcasses to improve pork quality. J. Anim. Sci. 76: 74 - 86.<br />PMid:9464887                                                          

</p><p class="p2"> <a name="Morales 2011"></a>Morales JI, Cámara L, Berrocoso JD, López JP, Mateos GG, Serrano MP (2011). Influence of sex and castration on growth performance and carcass quality of crossbred pigs from 2 Large White sire lines. J. Anim. Sci., 89 (11): 3481 - 3489.<br /><a href="http://dx.doi.org/10.2527/jas.2010-3357" target="_blank">http://dx.doi.org/10.2527/jas.2010-3357</a><br />PMid:21680787

</p><p class="p2"> <a name="Morcuende 2007"></a>Morcuende D, Estevez M, Ramirez R, Cava R (2007). Effect of the Iberian x Duroc reciprocal cross on productive parameters, meat quality and lipogenic enzyme activities. Meat Sci. 76: 86 – 94.<br /><a href="http://dx.doi.org/10.1016/j.meatsci.2006.10.016" target="_blank">http://dx.doi.org/10.1016/j.meatsci.2006.10.016</a><br />PMid:22064194                                                          

</p><p class="p2"> <a name="Oksbjerg  2002"></a>Oksbjerg N, Sørensen MT, Vestergaard M (2002). Compensatory growth and its effect on muscularity and technological meat quality in growing pigs. J. Anim. Sci., 52: 85 – 90.                                                      

</p><p class="p2"> <a name="Piao 2004"></a>Piao JR, Tian JZ, Kim BG, Choi YI, Kim YY, Han IK (2004). Effects of sex and market weight on growth performance carcass characteristics and pork quality of market hogs. Asian Australas. J. Anim. Sci. 10: 1452 –1458.

</p><p class="p2"> <a name="Roese 2006"></a>Roese G, Taylor G (2006). Basic pig husbandry — the weaner. Retrieved 26/04/14 from http://www.dpi.nsw.gov.au/__data/assets/pdf_file/0005/56147/Basic_pig_husbandry-The_weaner_-_Primefact_72-final.pdf                                                      

</p><p class="p2"> <a name="Serrano 2009"></a>Serrano MP, Valencia DG, Fuentetaja A, Lázaro R, Mateos GG  (2009). Effect of castration on productive performance, carcass characteristics and meat quality of Iberian pig females reared under intensive management systems. Livestock Sci.,   123: 147 – 153.<br /><a href="http://dx.doi.org/10.1016/j.livsci.2008.11.001" target="_blank">http://dx.doi.org/10.1016/j.livsci.2008.11.001</a>                                                      

</p><p class="p2"> <a name="Shipka 2011"></a>Shipka M (2011). Recommended practices for raising pigs from birth to weaning. Retrieved 13/08/13 from http://www.uaf.edu/files/ces/publications-db/catalog/anr/LPM-00845.pdf                                                      

</p><p class="p2"> <a name="Smith 2006"></a>Smith A, Stalder KJ, Serenius T, Baas T J, Mabry JW (2006). Effect of weaning age on nursery pig performance. Animal Industry Report: AS 652, ASL R2170. Retrieved 26/04/14 from http://lib.dr.iastate.edu/ans_air/vol652/iss1/76                                                      

</p><p class="p2"> <a name="Whiting 2008"></a>Whiting TL, Pasma T (2008). Isolated weaning technology: Humane benefits and concerns in the production of pork. Can. Vet. J. 49 (3): 293 - 301.<br />PMid:18390104    PMCid:PMC2249730                        



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