Research Article

Effects of Arbuscular Mycorrhizal Fungi on Spores Density and Root Colonization of Four Hybrids of Sunflower (Helianthus annuus L.) at Different Rock Phosphate Levels

Sayeda Sarah* and Muhammad Ibrar

Department of Botany, University of Peshawar, Khyber Pakhtunkhwa, Pakistan.

Abstract | The present experiment was carried out in a net house at the University of Peshawar, Pakistan. The main purpose of this research was to find out the effects of arbuscular mycorrhizal fungal (AMF) inoculation (with and without Mycorrhiza) along with application of various levels (0%, 25%, 50% and 100%) of rock phosphate (RP) fertilizer on spores density and percent root colonization of selected sunflower (Helianthus annuus L.) hybrids in P-deficient soil. It was observed that spore density and AMF root colonization was higher in the soil of control (RP0) plants, which decreases progressively with increasing fertility level. L ess number of spores and percent root colonization was found at high RP level (RP100) in all hybrids. Higher P doses declined the sporulation and colonization. There was total seven AMF species that were observed and recorded. The dominant genus was Acaulospora which was followed by Glomus, Sclerocystis and Gigaspora. The average AMF spore density ranged from 56-260 spores/100g soil while root colonization ranged from 32-100%. Mycorrhizal enhancement regarding AMF spores density and root colonization ranked as RP0>RP1>RP2>RP3 in all hybrids i.e 0%>25%>50%>100%.

Received | July 25, 2016 Accepted | August 25, 2016; Published | September 26, 2016

Correspondence | Sayeda Sarah, Department of Botany, University of Peshawar, Khyber Pakhtunkhwa, Pakistan; Email: syeda86@ymail.com

Citation | Sarah, S., and M. Ibrar. 2016. Effects of arbuscular mycorrhizal fungi on spores density and root colonization of four hybrids of sunflower (Helianthus annuus L.) at different rock phosphate levels. Sarhad Journal of Agriculture, 32(4): 258-266.

DOI | http://dx.doi.org/10.17582/journal.sja/2016.32.4.258.266

Keywords | AMF, Sunflower hybrids, Rock phosphate levels, AMF spores density, AMF root colonization

Introduction

Mycorrhiza is universal mutualistic associations between soil fungi and vascular plants and is essential in improving plant fitness and soil quality. These fungi improve the resilience of plant communities against environmental, nutritional and drought stresses (Barea et al., 2011). The AM fungi are known to be of great importance due to their high capability to increase growth, yield, and quality of crops through efficient nutrient acquisition in infertile soils and consequently lessening the prerequisite for Phosphate-based fertilizers (Khalafallah and Abo-Ghalia, 2008; Roy-Bolduc and Hijri, 2011). In turn, the fungi get carbon from the host plant. AM fungi have the capability of absorbing all essential macro and micro nutrients which are required for plant growth (Lester, 2009).

Sunflower (Helianthus annuus L.) belonging to the family Asteraceae is one of the important oilseed crops in the world and ranks fourth in production of vegetable oil (Weiss, 2000). About 18% of edible vegetable oil of the world is obtained from sunflower (Hussain et al., 2010). Sunflower production was 194,544 tons in 1998-99 which increased to 407224 tons in 2006-07.

Like most of the arid and semiarid soils of world soils of Pakistan are mostly Phosphorus (P) deficient due to their alkaline and calcareous nature, affecting plants adversely (Memon et al., 1992; NFDC, 2001). Total phosphorus contents of an average soil is about 0.05%, out of which only 0.1% of the total P is brought in use by plants because of its low solubility. To overcome this problem plants have adopted different strategies to acquire sufficient phosphorus (Sharma, 2004). Arbuscular mycorrhizal fungal association is one among these adaptations (Khade and Rodrigues, 2009; Coline et al., 2011). Due to scarcity of phosphorus reserves in the soil and their rapid utilization, efforts are being made to supplement plants with low grade rock phosphate. Mycorrhizal inoculation can help plants by solubilizing rock phosphate into available forms, which helps in plant growth (Sabanavar and Lakshman, 2009).

Throughout the world, scientists are focused on developing alternative technologies to minimize dependence on chemical fertilizers. Keeping the importance of AM fungi as bio-fertilizers, the present investigation was carried to find out the feasibility of inoculation of some high yielding sunflower hybrids with indigenous tropical AM fungi in low fertility soils, to achieve adequate production level.

Materials and Methods

Experimental Site

The present study was conducted at the Department of Botany, University of Peshawar, Pakistan.

Plant Material

Authentic seeds of four hybrids of sunflower i.e NKS-278, Hysun-33, SMH-0917 and SMH-0907 were obtained from Oil Seeds Research Program, NARC Islamabad, Pakistan.

Soil

The soil used was sandy loam with pH 7.8, electric conductivity 0.675dSm-1, Nitrogen 0.032% and Phosphorus 0.8 mgkg-1 with low organic matter (0.6%). All 96 pots having 89 cm diameter and 48 cm depth were filled with 6 Kg of this nutrient deficient soil.

Experimental Design and Treatments

The experimental work was carried out in a randomized complete block design (RCB) along with eight treatments; each treatment was replicated three times with five plants in each pot.

Application of AMF Inoculums

In the experimental work, mixed constorium of different AMF species i.e. Glomus fasciculatum, G.mosseae, G. aggregatum, Sclerocystis pakistanica, Gigaspora gigantea along with roots of wheat and maize infected with arbuscular mycorrhiza were used as rhizobase inoculum. The roots were cut into 1 cm pieces, which along with soil base inoculum (rhizospheric soil) were spread uniformly in pots, at a depth of 3 cm and 6 cm in layers before sowing. Inoculum for each pot consisted of 160 g of mycorrhizal infected roots and adhering soil. Mycorrhizal inoculum preparation, placement and application were done by the method given by Brundrett et al. (1996).

Fertilizer Application

Fertilizers were applied by following Krishna and Bagyaraj (1982) method. Rock phosphate fertilizer was obtained from Hazara deposits. Four levels of P fertilizer treatments 0 mg P2O5/kg soil, 100 mg P2O5/kg soil, 200 mg P2O5/kg soil and 500 mg P2O5/kg soil in form of RP were applied. The recommended dose was 80 kg P2O5/ha. These treatments were applied in combination with or without AMF.

Extraction of Spores

The spores were isolated from the soil samples by wet-sieving and decanting technique (Gerdemann and Nicolson, 1963).

Spore Density Calculation

Density of spores in each soil sample was calculated by following Stahl and Christensen (1982) standard method. The observed spores were micro photographed at two magnifications (4x, 10x).

Spores Identification

Spores were identified with the help of keys following Hall and Fish (1978), Trappe (1982) and Schenck and Perez (1990).

Assessment of Root Colonization

Roots were carefully dug out and washed thoroughly with water and stored in FAA (Formalin:Acetic acid:Alcohol) solution. The roots were stained and processed following the procedure of Phillips and Hayman (1970). For the assessment of root colonization + slide method of Giovannetti and Mosse (1980) was followed. Total of 25 segments of roots of individual plant each approximately 1 cm long were randomly selected for microscopic study. Morphology of AM entophyte was studied and expressed in percentage (%). The infection percentage was calculated by using the following formula (Giovannetti and Mosse, 1980):

Table 1: Effect of mycorrhiza on AMF spores in the roots of sunflower hybrids at various levels of rock phosphate (RP)

Rock Phosphate levels | Spores density / 100 g Soil
NKS-278 | Hysun- 33 | SMH-0917 | SMH-0907
RP0 (0%) | 202 | 260 | 251 | 246
RP1 (25%) | 171 | 202 | 182 | 171
RP2 (50%) | 103 | 172 | 145 | 122
RP3 (100%) | 56 | 102 | 88 | 79

Results and Discussion

AMF Spores Density

The results in Tables 1, 2 and Figure 1 shows the effect of various rock phosphate levels
on the AMF spores density in the rhizosphere soil of selected sunflower hybrids. Mycorrhizal enhancement regarding AMF spores density followed RP0>RP1>RP2>RP3 trend in all hybrids (Figure 1). It has been observed that spores density was higher in the soil of control (RP0) plants, which decreased progressively with increasing fertility level. Less number of spores was found at RP3 level in all hybrids. Generally the population of AMF spores and soil phosphorus are inversely related to each other (Hao et al., 1991). Chandrasekara et al. (2005) and Panwar and Tarafdar (2006) also found that interaction of mycorrhiza and phosphorus fertilizer had no significant effect on AMF spores density. Guillemin et al. (1995), Antunes et al. (2007) and Arumugam et al. (2010) reported that the spores density got declined sharply at high P level but these results negate the findings of Sharathbabu and Manoharachary (2006) who reported that dual inoculation of AMF (Glomus fasciculatum) and rock-phosphate significantly enhanced percentage of mycorrhizal colonization than in single inoculation or in control Tylophora indica plants.

Table 2:Effect of mycorrhiza on AMF spores species in sunflower hybrids

Percent Rock phosphate levels | AMF spores | Spores density in NKS-278 | Spores density in Hysun-33 | Spores density in SMH-0917 | Spores density in SMH-0907
RP0 (0%) | Acaulospora | +++ | +++ | +++ | +++
Sclerocystis | ++ | ++ | ++ | ++
Glomus | +++ | +++ | +++ | +++
Gigaspora | - | + | - | -
RP1 (25%) | Acaulospora | ++ | +++ | +++ | +++
Sclerocystis | - | ++ | + | -
Glomus | + | ++ | ++ | +
Gigaspora | - | - | + | -
RP2 (50%) | Acaulospora | + | +++ | ++ | +
Sclerocystis | - | + | + | -
Glomus | - | + | + | +
Gigaspora | - | - | + | -
RP3 (100%) | Acaulospora | - | + | - | -
Sclerocystis | - | - | - | -
Glomus | - | + | - | -
Gigaspora | - | - | - | -

+:Present; - :Absent; +++:100 %; ++:75 %; +:25 %

Average number of spores counted per 100 g of soil was different from hybrid to hybrid at various levels of treatments (Table 1 and Figure 1). The AMF spore densities ranged from 56-260 spores/ 100 g soil in selected sunflower hybrids. It was found that among control plants, the highest number of spores was recorded for Hysun-33 (260/100 g of soil) followed by SMH-0917 (251/100 g of soil), SMH-0907 (246/100 g of soil) and NKS-278 (202/100 g of soil). The combined effect of AMF+ RP results showed that AMF spores density followed Hysun-33>SMH-0917, SMH-0907> NKS-278 trend at all RP levels (Table 1).

AMF spores are ubiquitous in most ecosystems (Marleau et al., 2011), and are essential component of soil micro biota (Hindumathi and Reddy, 2011) AMF exists in soil as spores, hyphae, as vegetative propagules or infected root pieces for infecting plants, but mostly inoculation of plants is brought about by extraradical mycelium (ERM) (Sylvia and Jarstfer, 1992). Occurrence or distribution of AMF varies with host ranges (Sarwade et al., 2011).

AMF Species

Table 2 shows that the following species were identified in rhizosphere soil of selected sunflower hybrids at different rock phosphate levels (Figure 2).

- • Acalospora mellae (Spain and N.C. Schenck)
- • Acaulospora laevis (Gerd and Trappe, 1974)
- • Glomus fasiculatum (Thaxt. Gerd. and Trappe)
- • Glomus mosseae (T.H. Nicolson and Gerd)
- • Glomus aggregatum (N.C. Schenck and G.S. Sm.)
- • Sclerocystis pakistanica (S.H. Iqbal and Perveen)
- • Gigaspora gigantea (Gerd. and Trappe)

Nasim et al. (1998) showed that spores are the means of identification of these fungi. In the present work soil was collected from different pots with plants at reproductive stages of growth. Four genera of endogonaceous spores were identified which were Acaulospora (2 spp. i.e. A. mellae, A. laevis), Glomus (3 species i.e. G. mosseae, G. aggregatum and G. fasiculatum), Sclerocystis 1 specie i.e. (S. pakistanica) and Gigaspora (1 spp. G. gigantean) (Figure 2a, b, c).

In this research we found that the species of Acaulospora were most common and predominant followed by Glomus, Sclerocystis, and Gigaspora. Our findings are further supported by the work of other researchers (Lovelock et al., 2003; Wongmo, 2008; Tchabi et al., 2008; Charoenpakdee et al., 2010; Gao and Guo, 2010; Songachan and Kayang, 2011) who investigated that there is higher number of Acaulospora in the soil followed by Glomus species. The predominance of Acaulospora species might be due to their adaptation to wide variety of soil types, host species and pH and nutrient availability etc (Jefwa et al., 2006; Straker et al., 2010). It suggests that AMF strains are biological specific for the host plant as reported by Bever et al. (1996). The large number of AMF spores may be attributed to the deficiency of low phosphorus in the soil. Generally, the population of VAM spores and soil phosphorus are inversely related to each other (Hao et al., 1991).

The presence of small number of Gigasporaceae might be due to the fact that they are usually found in sandy dunes (Lee and Koske, 1994) and are usually large sized, which requires long period for their development than the small sized spores species (Hepper, 1984). Moreover, Gigaspora are very common in wild plants than field crops (Gai et al., 2006).

Table 3:Effects of various levels of RP fertilizers on REC index in the roots of sunflower hybrids

Rock phosphate levels | NKS-278 | Hysun-33 | SMH-0917 | SMH-0907
% age infection | % age infection | % age infection | % age infection
RP0 (0%) | 100% | 100% | 100% | 100%
RP1 (25%) | 72% | 96% | 91% | 88%
RP2 (50%) | 66% | 88% | 84% | 72%
RP3 (100%) | 32% | 46% | 42% | 35%

AMF Colonization in Roots

The results given in Tables 3 and 4 and Figure 3 show the effect of various rock phosphate levels on the percent root colonization in the rhizospheric soil of selected sunflower hybrids. Mycorrhizal enhancement regarding percent root colonization ranked as RP0>RP1>RP2>RP3 in all hybrids (Figure 3).

Table 4: Effects of various levels of RP fertilizers on AM infection Morphologies, external hyphae, internal hyphae, Arbuscules, vesicles, (%) in roots of mycorrhizal Sunflower hybrids

Percent AMF infection (%)
Hybrids | Treatments | External Hyphae (%) | Internal hyphae (%) | Vesicles (%) | Arbuscules (%)
NKS-278 | RP0 | 24±5.29 | 24.33±6.65 | 24±5.29 | 7.33±6.42
RP1 | 20±10 | 23.33±5.77 | 23.33±5.77 | 4±6.08
RP2 | 6.66±5.77 | 13.33±5.77 | 13.33±5.77 | *
RP3 | * | 3.33±5.77 | 6.66±11.54 | *
Hysun-33 | RP0 | 40.66±9.01 | 44±6.92 | 50.66±1.15 | 14±6.37
RP1 | 33.33±5.77 | 33.33±11.54 | 36.66±5.77 | 6.66±5.77
RP2 | 13.33±11.54 | 20±0 | 20±0 | 3.33±0
RP3 | * | 10±0 | 13.33±5.77 | *
SMH-0917 | RP1 | 40±10 | 43.33±5.77 | 50±0 | 7.33±6.42
RP0 | 23.33±5.77 | 23.33±5.77 | 23.33±5.77 | 3.33±6.35
RP2 | 10±10 | 16.66±5.77 | 20±0 | *
RP3 | * | 6.66±5.77 | 10±10 | *
SMH-0907 | RP0 | 36.66±1.54 | 37.33±5.77 | 40±0 | 13.33±5.94
RP1 | 20.66±10.06 | 24±6.92 | 24±5.29 | 4±6.92
RP2 | 3.33±5.77 | 10±0 | 10±0 | *
RP3 | * | 3.33±5.77 | 3.33±5.77 | *

±:Standard error; RP0:0% rock phosphate; RP1:25% rockphosphate; RP2:50% rock phosphate; RP3:100% rockphosphate

AMF root colonization was determined by the presence of external hyphae, internal hyphae, vesicles and arbuscules. The general AMF infection in sunflower hybrids at various rock phosphate levels was low as compared to control (RP0) viz 100% (Table 3). Vesicular infection was common and maximum at all RP levels in all hybrids (Figure 4)as reported by Iqbal and Bareen (1986) in Narcissus poeticus and Burni et al. (1993) in Targionia hypophylla. Moreover, Al-Raddad (1995) observed that the type of crop and harvesting greatly affect the root colonization. The comparison revealed that highest number of vesicles was recorded in Hysun-33 followed by SMH-0917, SMH-0907 and NKS-278 at all RP levels shown in Table 4, as reported by Linderman and Davis (2004) in marigold, Janoušková et al. (2007) for tobacco and Sensoy et al. (2007) for Capsicum annuum L.

The results (Table 3 and 4) shows that AMF inoculated plants had significant positive effects on AMF root colonization. However, this positive effect of AMF inoculated plants decreased with increasing RP level; lowest root colonization was found at RP3 level in all hybrids. Redecker (2005) found that high concentration of phosphate seems to induce low fungal colonization level by the plants.

It was found that AMF colonization was higher in the control plants of all hybrids whereas it decreased to a minimum 32% in NKS-278, 35% in SMH-0907, 42% in SMH-0917 and 46% in Hysun-33 at high P levels (RP3) (Table 3, Figures 3 and 4). These results agreed the results of Soleimanzadeh (2012) who showed that positive effect of AMF colonization decreases with increasing P levels. Similar results were reported by Chandrashekara et al. (1995), Mohammad et al. (2003) and Pragatheswari et al. (2004). This might be attributed to the fact that low phosphorus result in exudation of certain chemicals from the root which enhances AMF colonization and spore germination but such exudations does not take place when phosphorus level is high (Juniper and Abbott, 2006; Murkute et al., 2009).

Sharif et al. (2011) and Manske (1990) showed that low availability of soil phosphorus increases AMF colonization. But, results of Satpal and Kapoor (2000) showed that dual inoculation of Vigna radiata plants with rock phosphate and AMF stimulated root colonization as compared to those without rock phosphate. The results also shows that at control and low level of rock phosphate the internal hyphae and arbuscular infection were moderately frequent and scattered throughout the cortex which is actually the sites of nutrients exchange (Table 4) . The external hyphae and arbuscules were not seen in any of the studied root segments at high RP level (RP3) as shown in Table 4. High soil phosphate level has direct effect on reduction of hyphal growth and spore production.

Conclusion

This study clearly indicates the potential of using indigenous biofertilizer such as AMF for oil seed crops in low fertility soils, to achieve adequate production level with least utilization of synthetic fertilizers for sustainable agriculture practice. The use of biofertilizer is not only eco-friendly but also economical as it reduces our dependence on expensive chemical fertilizers.

Authors’ Contribution

Prof. Dr. M. Ibrar has supervised Ms. Sayeda Sarah in her PhD program and this article is a portion of the research.

References

Al-Raddad, A.M. 1995. Mass production of Glomus mosseae spores. Mycorrhiza. 5:229-231. http://dx.doi.org/10.1007/BF00203343

Antunes, P.M., K. Schneider, D. Hillis and J.N. Klironomos. 2007. Can the arbuscular mycorrhizal fungus Glomus intraradices actively mobilize P from rock phosphates? Pedobiologia. 51(4):281-286. http://dx.doi.org/10.1016/j.pedobi.2007.04.007

Arumugam, R., S. Rajasekaran and S.M. Nagarajan. 2010. Response of Arbuscular Mycorrhizal fungi and rhizobium Inoculation on growth and chlorophyll content of Vigna unguiculata (L) Walp Var. Pusa 151. J. Appl. Sci. Environ. Manage. 14(4):113-115.

Barea, J.M., J. Palenzuela, P. Cornejo, I. Sanchez-Castro, C. Navarro-Fernandez, A. Lopez-García, B. Estrada, R. Azcon, N. Ferrol and C. Azcon-Aguilar. 2011. Ecological and functional roles of mycorrhizas in semi-arid ecosystems of Southeast Spain, J. Arid Environ. 75(12):1292-1301. http://dx.doi.org/10.1016/j.jaridenv.2011.06.001

Bever, J.D., J.B. Morton, J. Antonovics and P.A. Schultz. 1996. Host-dependent sporulation and species diversity of arbuscular mycorrhizal fungi in mown grassland. J. Ecol. 84:71–82. http://dx.doi.org/10.2307/2261701

Brundrett, M., N. Bougher, B. Dell, T. Grove and N. Malajazuk. 1996. Working with mycorrhizas in forestry and agriculture. Monograph 32, Australian Centre for International Agricultural Research, Canberra, Australia.

Burni, T., Z. Muhammad and A. Hussain. 1993. VAM association in Targionia hypophylla. Sci. Khy. 6:65-70.

Chandrasekara, C.M.C.P., H.M.S.P.M. Weerasinghe, I.A.U.N. Gunatilleke and G. Seneviratne. 2005. Spatial distribution of arbuscular mycorrhizas along an elevation and adaphic gradient in the forest dynamics plot at Sinharaja, Srilanka. Cey. J. Sci. 34:47-67.

Chandrashekara, C.P., V.C. Patil and M.N. Sreenivasa. 1995. VA-mycorrhiza mediated P effect on growth and yield of sunflower (Helianthus annuus L.) at different P levels. Plant Soil. 176(2):325-328. http://dx.doi.org/10.1007/BF00011797

Charoenpakdee, S., P. Cherdchai, B. Dell and S. Lumyong. 2010. The mycorrhizal status of indigenous arbuscular mycorrhizal fungi of physic nut (Jatropha curcas) in Thailand. Mycosphere. 1(2):167– 181.

Coline, B., V. Puech-Page, G. Becard and S.F. Rochange. 2011. The regulation of arbuscular mycorrhizal symbiosis by phosphate in pea involves early and systemic signalling events. J. Exp. Bot. 62(3):1049–1060. http://dx.doi.org/10.1093/jxb/erq335

Gai, J.P., G.X. Feng, B. Cai, P. Christie and X.L. Li. 2006. A preliminary survey of the arbuscular mycorrhizal status of grassland plants in southern Tibet. Mycorrhiza. 16:191–196. http://dx.doi.org/10.1007/s00572-005-0032-7

Gao, Q.M. and L.D. Guo. 2010. A comparative study of arbuscular mycorrhizal fungi in forest, grassland and cropland in the Tibetan Plateau, China. Mycol. 1:163–170. http://dx.doi.org/10.1080/21501203.2010.510123

Gerdemann, J.W. and T.H. Nicholson. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Trans. Br. Mycol. Soc. 46:235-244. http://dx.doi.org/10.1016/S0007-1536(63)80079-0

Giovannetti, M. and B. Mosse. 1980. Evaluation techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol. 133:45-57. http://dx.doi.org/10.1111/j.1469-8137.1980.tb04556.x

Guillemin, J.P., M.O. Orozco, V. Gianinazzi-Pearson and S. Gianinazzi. 1995. Influence of phosphate fertilization on fungal alkaline phosphatase and succinate dehydrogenase activities in arbuscular mycorrhiza of soybean and pineapple. Agric. Ecosys. Environ. 53:63–69. http://dx.doi.org/10.1016/0167-8809(94)00555-S

Hall, I.R., and B.J. Fish. 1978. A key to the Endogonaceae. Trans. Br. Mycol. Soc. 73:261-270. http://dx.doi.org/10.1016/S0007-1536(79)80110-2

Hao, W.Y., X.G. Lin, X.X. Gu and J.Q. Niu. 1991. Efficiency of VAM fungi and the prospect of their practical application in some soils. Nanjing Inst. Soil. Sci. 28(2):129-131.

Hepper, C.M. 1984. Isolation and culture of VA mycorrhizal (VAM) fungi. In:VA Mycorrhizae. (Eds. CL Powell, DJ Bagyaraj) CRC Press, Florida, USA. Pp.95-112.

Hindumathi, A. and B.N. Reddy. 2011. Occurrence and distribution of arbuscular mycorrhizal fungi and microbial flora in the rhizosphere soils of mungbean [Vigna radiata (L.) wilczek] and soybean [Glycine max (L.) Merr.] Adilabad, Nizamabad and Karimnagar districts of Andhra Pradesh state, India. Adv. Biosci. Biotech. 2:275-286. http://dx.doi.org/10.4236/abb.2011.24040

Hussain, M., M. Farooq, K. Jabran, and A. Wahid. 2010. Foliar application of glycinebetaine and salicylic acid improves growth, yield and water productivity of hybrid sunflower planted by different sowing methods. J. Agron. Crop Sci. 196:136-145. http://dx.doi.org/10.1111/j.1439-037X.2009.00402.x

Iqbal, S.H., and F. Bareen. 1986. Morphogenisis of underground part of field grown Narcissus poeticus L., in relation to VA mycorrhizal infection. Biologia. 32(2):371-381.

Janouskova, M., M. Vosátka, L. Rossi and N. Lugon-Moulin. 2007. Effects of arbuscular mycorrhizal inoculation on cadmium accumulation by different tobacco (Nicotiana tabacum L.) types. Appl. Soil Ecol. 35:502-510. http://dx.doi.org/10.1016/j.apsoil.2006.10.002

Jefwa, J.M., R. Sinclair and J.A. Maghembe. 2006. Diversity of glomale mycorrhizal fungi in maize/sesbania intercrops and maize monocrop systems in southern Malawi. Agroforestry Sys. 67(2):107-114. http://dx.doi.org/10.1007/s10457-004-2370-4

Juniper, S. and L.K. Abbott. 2006. Soil salinity delays germination and limits growth of hyphae from propagules of arbuscular mycorrhizal fungi. Mycorrhiza. 16:371-379. http://dx.doi.org/10.1007/s00572-006-0046-9

Khade, S.W., and B.F. Rodrigues. 2009. Studies on effects of arbuscular mycorrhizal (AM) fungi on mineral nutrition of Carica papaya L. Not. Bot. Horti. Agrobot. Cluj Napoca. 37(1):183-186.

Khalafallah, A.A., and H.H. Abo-Ghalia. 2008. Effect of arbuscular mycorrhizal fungi on the metabolic products and activity of antioxidant system in wheat plants to short term water stress, followed by recovery at different growth stages. J. Appl. Sci. Res. 4(5):559-569.

Krishna, K.R., and D.J. Bagyaraj. 1982. Interaction between a vesicular arbuscular fungus and Streptomyces cinnamomeous and their effects on finger millet. New Phytol. 92:401-405. http://dx.doi.org/10.1111/j.1469-8137.1982.tb03397.x

Lee, P.J., and R.E. Koske. 1994. Gigaspora gigantia:Seasonal, abundance and ageing of spores in a sand dune. Mycologic. Res. 98:453-457. http://dx.doi.org/10.1016/S0953-7562(09)81203-3

Lester, D. 2009. Buying and applying mycorrhizal fungi. Max. Yield. USA. Pp. 126-131. (http://www.maximumyield.com/article_sh db.php?articleID=483).

Linderman, R.G., and A.E. Davis. 2004. Vaired response of marigold (Tagetes spp.) genotypes to inoculation with different arbuscular mycorrhizal fungi. Sci. Hort. 99:67-78. http://dx.doi.org/10.1016/S0304-4238(03)00081-5

Lovelock, C.E., K. Andersen and J.B. Morton. 2003. Arbuscular mycorrhizal communities in tropical forests are affected by host tree species and environment. Oecologia. 135:268-279. http://dx.doi.org/10.1007/s00442-002-1166-3

Manske, G.G.B. 1990. Genetical analysis of efficiency of VAM with spring wheat. Agric. Eco. Envir. 29(14):273-280. http://dx.doi.org/10.1016/0167-8809(90)90287-N

Marleau, J., Y. Dalpe, M. St-Arnaud and M. Hijri. 2011. Spore development and nuclear inheritance in arbuscular mycorrhizal fungi. BMC Evol. Biol. 11:51. http://dx.doi.org/10.1186/1471-2148-11-51

Memon, K.S., A. Rashid and H.K. Puno. 1992. Phosphorus deficiency diagnosis and P soil test calibration in Pakistan. Trop. Soil Bulletin. 92(2):125-147.

Mohammad, M.J., S.R. Hamad and H.I. Malkawi. 2003. Population of arbuscular mycorrhizal fungi insemi-aridenvironment of Jordan as influenced by biotic and abiotic factors. J. Arid Environ. 53:409-417. http://dx.doi.org/10.1006/jare.2002.1046

Murkute, A.A., S. Sharma, S.K. Singh and V.B. Patel. 2009. Response of mycorrhizal citrus rootstock plantlets to salt stress. Indian J. Hort. 66:456-460.

Nasim, G., S. Saeed, M. Shaheen, Z.H. Naqui and S. Sheikh. 1998. Wheat stumps:A source of VAM inoculum for the incoming crop. Scientific Khyber. 11(2):43-56.

NFDC. 2001. Balanced fertilization through phosphate promotion. Project terminal report NFDC, Islamabad, Pakistan.

Panwar, J. and J.C. Tarafdar. 2006. Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. J. Arid Environ. 65(3):337-350. http://dx.doi.org/10.1016/j.jaridenv.2005.07.008

Phillips, J.M., and D.S. Hayman. 1970. Improved procedure for clearing root parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Brit. Mycol. 55:158-161. http://dx.doi.org/10.1016/S0007-1536(70)80110-3

Pragatheswari, D., A. Manjunath, M. Madhaiyan, K. Kumutha. 2004. Soil solution phosphorus status and mycorrhizal inoculation efficiency of selected tropical grain
legumes in an alfisol. Jodhpur, India:Scientific Publishers (India). Biofertilizers
technology for rice based cropping system. p. 334-341.

Redecker, D. 2005. Glomeromycota. AM Fungi and their relatives. In the tree of web projects. http. ll tolweb. org/ Glomeromycota/28715.

Roy-Bolduc, A., and M. Hijri. 2011. The use of mycorrhizae to enhance phosphorus uptake:A way out the phosphorus crisis. J. Biofertil. Biopestici. 2:104.

Sabanavar, S.J., and H.C. Lakshman. 2009. Effect of Rock Phosphate solubilization using Mycorrhizal fungi and Phosphobaacteria on two high yielding varities of Sesamum indicum L. World J. Agri. Sci. 5(4):470-479.

Sarwade, P.P., S.S. Chandanshive, M.B. Kanade and U.N. Bhale. 2011. Diversity of Arbuscular mycorrhizal (AM) fungi in some common plants of marathwada region. Int. Multidiscipl. Res. J. 1(12):11-12.

Satpal, S., and K.K. Kapoor. 2000. Influence of inoculation of different vesicular arbuscular mycorrhizal fungi on growth and nutrient of mungbean and wheat. Manila, Philippines:Science and Technology Information Institute. Philip. J. Sci. 129(1):19-25.

Schenck, N.C., and Y. Perez. 1990. Manual for the Identification of VAM Fungi. 3rd Ed. University of Florida, Gainesville. U.S.A. p. 1-283.

Sensoy, S., S. Demir, O. Turkmen, C. Erdinc and O.B. Savur. 2007. Responses of some different pepper (Capsicum Annuum L.) genotypes to inoculation with two different arbuscular mycorrhizal fungi. Sci. Hort. 113:92-95. http://dx.doi.org/10.1016/j.scienta.2007.01.023

Sharathbabu, K., and C. Manoharachary. 2006. Impact of AM fungi and Rock-phosphate on mycorrhizal colonization, growth and nutrition of Tylophora indica (Burm. f.) Merril. Under glass house conditions. Indian Phytopath. 59(4):427-431 .

Sharif, M., E. Ahmad, M.S. Sarir, D. Muhammad, M. Shafi and J. Bakht. 2011. Response of different crops to arbuscular mycorrhiza fungal inoculation in phosphorus-deficient soil. Commun. Soil Sci. Plant Anal. 42(9):2299-2309. http://dx.doi.org/10.1080/00103624.2011.605488

Sharma, A.K. 2004. Biofertilizer for sustainable Biological mobilization of phosphorus 1st Ed. Pp. 206.

Soleimanzadeh. 2012. Response of Sunflower (Helianthus annuus L.) to Inoculation with mycorrhiza under different phosphorus levels. Am-Eurasian J. Agric. Environ. Sci. 12(3):337-341.

Songachan, L.S., and H. Kayang. 2011. Diversity of arbuscular mycorrhizal fungi in pine forest of Meghalaya, North East India. Mycosphere. 2(4):497-505.

Stahl, P.D., and M. Christensen. 1982. Mycorrhizal fungi associated with Bouteloua and Agropyron in Wyoming sagebrush-grasslands. Mycologia. 74:877-885. http://dx.doi.org/10.2307/3792716

Straker, C.J., A.J. Hilditch and M.E.C. Rey. 2010. Arbuscular mycorrhizal fungi associated with cassava (Manihot esculenta Crantz). South Afr. J. Bot. 76:102–111. http://dx.doi.org/10.1016/j.sajb.2009.09.005

Sylvia, D.M., and A.G. Jarstfer. 1992. Sheared root inocula of vesicular-arbuscular mycorrhizal fungi. Appl. Environ. Microbial. 58:229-232.

Tchabi, A., C. Danny, H. Fabien, L. Lousi, W. Andres and O. Fritz. 2008. Arbuscular mycorrhizal fungal communities in sub-Saharan Savannas of Benin, West Africa, as affected by agricultural land use intensity and ecological zone. Mycorrhiza. 18:185–191. http://dx.doi.org/10.1007/s00572-008-0171-8

Trappe, J.M. 1982. Synoptic key to the genus and species of zygomycetous mycorrhizal fungi. Phytopathol. 72:1102-1108.

Weiss, E.A. 2000. Oil seed crops. Blackwell Sci. Led. London. p. 364.

Wongmo, J. 2008. Influences of arbuscular mycorrhizal fungi on different food crops. Ph.D. Thesis, Chiang Mai University, Chiang Mai, Thailand.